Photocatalyst Group, Kanagawa Academy of Science and Technology, Sakado, Takatsu-ku, Kawasaki, Japan.
Photochem Photobiol Sci. 2011 Nov;10(11):1825-9. doi: 10.1039/c1pp05192j. Epub 2011 Sep 20.
The International Organization for Standardization (ISO) was used to evaluate antibacterial activity by titanium dioxide (TiO(2)) photocatalysis since 2006. We evaluated photocatalytic inactivation of Qβ and T4 bacteriophages induced by low-intensity, long-wavelength ultraviolet A (UVA; 0.1 mW cm(-2) and 0.001 mW cm(-2)) irradiation on a TiO(2)-coated glass plate using the ISO methodology. The results indicated that both bacteriophages were inactivated at 0.001 mW cm(-2) UVA. The intensity of UV light, including long-wavelength light (UVA), is very low in an actual indoor environment. Thus, TiO(2) photocatalysis can be beneficial for inactivating viruses in an indoor environment. Experiments using qPCR and bovine serum albumin degradation assume that viral inactivation is caused by outer viral protein disorder and not by viral RNA reduction by reactive oxygen species produced during TiO(2) photocatalysis. Furthermore, we showed that the ISO methodology for standard testing of antibacterial activity by TiO(2) photocatalysis can be applied to assess antiviral activity.
自 2006 年以来，国际标准化组织（ISO）一直用于评估二氧化钛（TiO2）光催化的抗菌活性。我们使用 ISO 方法评估了在 TiO2 涂层玻璃板上用低强度、长波长紫外线 A（UVA；0.1 mW cm(-2) 和 0.001 mW cm(-2))照射诱导的 Qβ和 T4噬菌体的光催化失活。结果表明，两种噬菌体在 0.001 mW cm(-2) UVA 下均被失活。实际室内环境中的紫外线光（包括长波长光 UVA）的强度非常低。因此，TiO2 光催化作用有助于在室内环境中灭活病毒。使用 qPCR 和牛血清白蛋白降解的实验假设病毒失活是由病毒外蛋白的无序引起的，而不是由 TiO2 光催化作用产生的活性氧还原病毒 RNA 引起的。此外，我们表明，TiO2 光催化抗菌活性的 ISO 标准测试方法可用于评估抗病毒活性。
