Wu Xuan, Liu Hongchen, E Lingling, Li Zhenchun
Dept. of Prosthodontics, Stomatology Hospital of Dalian City, Dalian 116021, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Aug;29(4):348-50, 354.
To explore the expression of glucose transporter (GLUT)-1 and insulin receptor (IR) alpha1 in osteoblast obtained from diabetic rats' mandibles.
The expression of GLUT-1 and IR alpha1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain.
The mRNA expressions of GLUT-1 and IR alpha1 of diabetic group were significantly higher than control group (P<0.05). The protein expressions of GLUT-1 and IR alpha1 were similar to the mRNA expressions.
Osteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.
探讨糖尿病大鼠下颌骨成骨细胞中葡萄糖转运蛋白（GLUT）-1和胰岛素受体（IR）α1的表达情况。
采用逆转录聚合酶链反应、蛋白质免疫印迹法和免疫组织化学染色法检测糖尿病组和对照组中GLUT-1和IRα1的表达。
糖尿病组GLUT-1和IRα1的mRNA表达明显高于对照组（P<0.05）。GLUT-1和IRα1的蛋白表达与mRNA表达相似。
糖尿病大鼠下颌骨成骨细胞对高血糖和低胰岛素血症保持适应性变化，这可能导致其功能障碍。
