[S100A9在糖尿病大鼠牙周组织中的表达及作用]
[Expression and effect of S100A9 in the periodontium of diabetic rats].
作者信息
Wenhua Sun, Guoqing Chen, Weidong Tian
机构信息
College of Life Science, Sichuan University, Chengdu 610065, China;State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Aug 1;34(4):419-424. doi: 10.7518/hxkq.2016.04.020.
OBJECTIVE
The study seeks to investigate the expression of S100A9 and its potential role in periodontal diseases induced by diabetes.
METHODS
A diabetic SD rat model was established through intraperitoneal injection of streptozotocin (STZ). Hematoxylin-eosin (HE) staining was performed to study the structure of the periodontium of diabetic rats. Using immunohistochemical staining, the distribution of S100A9 expression was detected in the periodontium of diabetic rats. Ex-pressions of Toll-like receptor 4 (TLR4) (ligands of S100A9) and p-P65/nuclear factor κB (NF-κB) were also measured.
RESULTS
The trabecular structure of alveolar bone was sparser, and lamina dura was disappeared in diabetic rats. Obviously higher expressions of S100A9 were observed in the periodontal ligament, alveolar bone, and gingival epithelial of diabetic rats than in the control rats. TLR4 expressions in the periodontal ligament, alveolar bone and gingival epithelial of the diabetic rats were also higher as compared to the control rats. p-P65 expression was not detected in the control rats, but was detected in the periodontal ligament and alveolar bone of the diabetic rats.
CONCLUSIONS: Periodontium lesions in diabetes mellitus may be induced by the activation of TLR4 and NF-κB signaling pathway meditated by S100A9. .
目的
本研究旨在探讨S100A9的表达及其在糖尿病所致牙周疾病中的潜在作用。
方法
通过腹腔注射链脲佐菌素（STZ）建立糖尿病SD大鼠模型。采用苏木精-伊红（HE）染色观察糖尿病大鼠牙周组织的结构。运用免疫组织化学染色检测糖尿病大鼠牙周组织中S100A9表达的分布情况。同时检测Toll样受体4（TLR4）（S100A9的配体）和p-P65/核因子κB（NF-κB）的表达。
结果
糖尿病大鼠牙槽骨小梁结构稀疏，硬骨板消失。糖尿病大鼠牙周膜、牙槽骨和牙龈上皮中S100A9的表达明显高于对照组大鼠。糖尿病大鼠牙周膜、牙槽骨和牙龈上皮中TLR4的表达也高于对照组大鼠。对照组大鼠未检测到p-P65表达，而糖尿病大鼠的牙周膜和牙槽骨中检测到p-P65表达。
结论
糖尿病中的牙周组织病变可能由S100A9介导的TLR4和NF-κB信号通路激活所致。
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