细胞角蛋白17在口腔鳞状细胞癌中的表达
[Expression of cytokeratin 17 in oral squamous cell carcinoma].
作者信息
Wei Kuijie, Zhang Lei, Yang Xiao, Han Wei, Zhong Laiping, Ye Dongxia, Zhiyuan Zhang
机构信息
Dept. of Stomatology, School of Medicine, Huzhou Normal University, Huzhou 313000, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Aug;29(4):404-8.
OBJECTIVE
To investigate the expression of cytokeratin 17 (CK17) in oral squamous cell carcinoma (OSCC) as well as its clinical significance.
METHODS
Detection of the mRNA level and protein expression of CK17 in the in vitro cellular carcinogenesis model of OSCC, some OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blot and immunohistochemistry, respectively.
RESULTS
Increased CK17 mRNA level was observed in the HB56 and OSC cell lines compared with the HIOEC using real-time PCR technique. Western blot showed increased CK17 protein expression in all the cell lines compared with the HIOEC. Increased CK17 mRNA and immunoreaction levels were also observed in the cancerous tissue specimens from OSCC patients compared with normal adjacent tissues (P<0.01).
CONCLUSION
The significantly increased CK17 gene may be associated with the tumorigenesis and development of OSCC.
目的
探讨细胞角蛋白17（CK17）在口腔鳞状细胞癌（OSCC）中的表达及其临床意义。
方法
分别采用实时聚合酶链反应（PCR）、蛋白质印迹法和免疫组织化学法，检测OSCC体外细胞癌变模型、部分OSCC细胞系以及30例原发性OSCC患者组织标本中CK17的mRNA水平和蛋白表达。
结果
运用实时PCR技术观察到，与永生化人正常口腔黏膜上皮细胞（HIOEC）相比，HB56和OSC细胞系中CK17的mRNA水平升高。蛋白质印迹法显示，与HIOEC相比，所有细胞系中CK17蛋白表达均增加。与癌旁正常组织相比，OSCC患者癌组织标本中CK17的mRNA和免疫反应水平也升高（P<0.01）。
结论
CK17基因显著上调可能与OSCC的发生发展有关。
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