Chen Tao, Wang Yanhui, Bu Lingxue, Li Ningyi
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Aug;29(4):442-5.
To construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.
Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.
When temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.
Cell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.
采用细胞片层技术与传统骨组织工程方法构建功能性组织工程骨。
采用密度梯度离心法分离犬骨髓间充质干细胞（BMSCs）并进行培养。将BMSCs诱导分化为成骨细胞，在温度响应培养皿中于37℃、5%二氧化碳及饱和湿度条件下培养。当温度降至20℃时制备BMSCs细胞片层。制备脱矿骨基质（DBM）和富血小板血浆（PRP），构建DBM/PRP/BMSCs细胞片层/BMSCs复合物并植入左背阔肌下。将DBM/PRP/BMSCs复合物植入右背阔肌下。
当温度降至20℃时，BMSCs自动从温度响应培养皿上脱落并形成完整的细胞片层。DBM/PRP/BMSCs细胞片层/BMSCs组的成骨能力优于DBM/PRP/BMSCs组。
细胞片层技术与传统骨组织工程相结合为构建理想的功能性组织工程骨提供了一种新方法。
