槲皮素对器官培养小鼠蝶枕结合部 Sox9 表达及随后 II 型胶原释放的影响。
The effect of quercetin on expression of SOX9 and subsequent release of type II collagen in spheno-occipital synchondroses of organ-cultured mice.
机构信息
University of Hong Kong, Hong Kong.
出版信息
Angle Orthod. 2012 Mar;82(2):247-53. doi: 10.2319/042111-278.1. Epub 2011 Sep 20.
OBJECTIVE
To identify the expressions of SOX9 and type II collagen in spheno-occipital synchondrosis in response to quercetin, using a mouse in vitro model.
MATERIALS AND METHODS
A total of 50 one-day-old male BALB/c mice were randomly assigned to the control and experimental groups. Each group was subdivided into five different time points, which were 6, 24, 48, 72, and 168 hours, and each subgroup contained 5 mice (n = 5). In the experimental group, the spheno-occipital synchondrosis was immersed in the BGJb medium + quercetin dihydrate 1 µM. In the control group, the spheno-occipital synchondrosis was immersed in the BGJb medium. Tissue sections were subjected to immunohistochemical staining for SOX9 and type II collagen expressions.
RESULTS
Quantitative analysis revealed there was a statistically significant increase of 32.31% (P < .001) in the expression of SOX9 between experimental groups and control groups at 24 hours. Furthermore, there was a statistically significant increase of 22.99% (P < .001) in the expression of type II collagen between experimental groups and control groups at 72 hours.
CONCLUSION
The expressions of SOX9 and type II collagen in the spheno-occipital synchondrosis can be increased by quercetin.
目的
使用体外小鼠模型，研究槲皮素对蝶枕结合部的 SOX9 和 II 型胶原表达的影响。
材料和方法
将 50 只 1 日龄雄性 BALB/c 小鼠随机分为对照组和实验组，每组再分为 6、24、48、72 和 168 小时 5 个不同时间点亚组，每个时间点亚组包含 5 只小鼠（n = 5）。实验组将蝶枕结合部浸泡于 BGJb 培养基+槲皮素二水合物 1 µM 中，对照组将蝶枕结合部浸泡于 BGJb 培养基中。对组织切片进行 SOX9 和 II 型胶原表达的免疫组织化学染色。
结果
定量分析显示，在 24 小时时实验组与对照组的 SOX9 表达增加了 32.31%（P <.001），具有统计学意义；在 72 小时时实验组与对照组的 II 型胶原表达增加了 22.99%（P <.001），具有统计学意义。
结论
槲皮素可增加蝶枕结合部的 SOX9 和 II 型胶原的表达。
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