人类单核细胞中多酚槲皮素的全基因组定位。
Genome-wide localization of the polyphenol quercetin in human monocytes.
机构信息
Department of Oxidative Stress and Human Diseases, MIGAL - Galilee Research Institute, 11016, Kiryat Shmona, Israel.
Faculty of Medicine in the Galilee, Bar-Ilan University, 1311502, Safed, Israel.
出版信息
BMC Genomics. 2019 Jul 23;20(1):606. doi: 10.1186/s12864-019-5966-9.
BACKGROUND
Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action at the genome-wide level. The present study aims to characterize quercetin's interaction with cellular DNA and to show its subsequent effect on downstream transcription.
RESULTS
Two massive parallel DNA-sequencing technologies were used: Chem-seq and RNA-seq. We demonstrate that upon binding to DNA or genome-associated proteins, quercetin acts as a cis-regulatory transcription factor for the expression of genes that are involved in the cell cycle, differentiation and development.
CONCLUSIONS
Such findings could provide new and important insights into the mechanisms by which the dietary polyphenol quercetin influences cellular functions.
背景
槲皮素是一种多酚，具有抗氧化活性和参与免疫反应的特性，因此备受关注。尽管槲皮素在分子水平上作为基因调节剂和特定细胞途径的激活剂已得到充分研究，但在基因组水平上其作用机制尚未得到太多关注。本研究旨在描述槲皮素与细胞 DNA 的相互作用，并展示其对下游转录的后续影响。
结果
使用了两种大规模平行 DNA 测序技术：Chem-seq 和 RNA-seq。我们证明，槲皮素与 DNA 或与基因组相关的蛋白质结合后，可作为涉及细胞周期、分化和发育的基因表达的顺式调控转录因子。
结论
这些发现可能为膳食多酚槲皮素影响细胞功能的机制提供新的重要见解。
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