Institute of Pathology, Rheinisch-Westfälische Technische Hochschule Aachen University, Aachen, Germany.
PLoS One. 2011;6(9):e23195. doi: 10.1371/journal.pone.0023195. Epub 2011 Sep 14.
Mesenchymal stem cells (MSC) represent a particularly attractive cell type for bone tissue engineering because of their ex vivo expansion potential and multipotent differentiation capacity. MSC are readily differentiated towards mature osteoblasts with well-established protocols. However, tissue engineering frequently involves three-dimensional scaffolds which (i) allow for cell adhesion in a spatial environment and (ii) meet application-specific criteria, such as stiffness, degradability and biocompatibility.
METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we analysed two synthetic, long-term degradable polymers for their impact on MSC-based bone tissue engineering: PLLA-co-TMC (Resomer® LT706) and poly(ε-caprolactone) (PCL). Both polymers enhance the osteogenic differentiation compared to tissue culture polystyrene (TCPS) as determined by Alizarin red stainings, scanning electron microscopy, PCR and whole genome expression analysis. Resomer® LT706 and PCL differ in their influence on gene expression, with Resomer® LT706 being more potent in supporting osteogenic differentiation of MSC. The major trigger on the osteogenic fate, however, is from osteogenic induction medium.
This study demonstrates an enhanced osteogenic differentiation of MSC on Resomer® LT706 and PCL compared to TCPS. MSC cultured on Resomer® LT706 showed higher numbers of genes involved in skeletal development and bone formation. This identifies Resomer® LT706 as particularly attractive scaffold material for bone tissue engineering.
间充质干细胞（MSC）因其体外扩增潜力和多能分化能力，成为骨组织工程中特别有吸引力的细胞类型。MSC 很容易通过成熟的方案向成熟的成骨细胞分化。然而，组织工程经常涉及三维支架，这些支架（i）允许细胞在空间环境中附着，（ii）满足特定应用的标准，如刚度、可降解性和生物相容性。
方法/主要发现：在本研究中，我们分析了两种用于基于 MSC 的骨组织工程的合成、长期可降解聚合物：聚丙交酯-co-三甲撑碳酸酯（Resomer® LT706）和聚（ε-己内酯）（PCL）。与组织培养聚苯乙烯（TCPS）相比，这两种聚合物通过茜素红染色、扫描电子显微镜、PCR 和全基因组表达分析均增强了 MSC 的成骨分化。Resomer® LT706 和 PCL 对基因表达的影响不同，Resomer® LT706 在支持 MSC 的成骨分化方面更有效。然而，成骨命运的主要触发因素是成骨诱导培养基。
与 TCPS 相比，MSC 在 Resomer® LT706 和 PCL 上的成骨分化增强。在 Resomer® LT706 上培养的 MSC 显示出更多涉及骨骼发育和骨形成的基因。这表明 Resomer® LT706 是骨组织工程中特别有吸引力的支架材料。
