Ergin Cağrı, Akkaya Yüksel, Kiriş Satılmış Ozgün, Yılmaz Cansev
Pamukkale University Faculty of Medicine, Department of Medical Microbiology, Denizli, Turkey.
Mikrobiyol Bul. 2011 Jul;45(3):461-7.
The laboratory diagnosis of Bartonella henselae infection is mainly based on serological testing by indirect immunofluorescence assay (IFA). Cell line co-cultivation with B.henselae and agar derivated antigens are the two major procedures used for evaluation of anti-Bartonella antibodies. Vero and Hep-2 cell lines are the most commonly used media for co-cultivation both in-house and commercial diagnostic kits production. However, HeLa cells which are easily supplied and grown, also can easily be infected by B.henselae. The aim of this study was to compare the performances of antigens obtained by co-cultivation of B.henselae ATCC 49882 (Houston-1) in Vero and HeLa Cells in IFA serology. Out of 381 sera samples, 127 (33.3%) were found positive and 195 (51.2%) were found negative by IFA performed by both cell line co-cultivations. The total agreement between the methods were found as 84.5% (322/381), and Cohen kappa value was calculated as 0.68 (strong, coherent). As a result, He-La cells were found to be useful for the preparation of B.henselae antigens to be used in IFA for the serologic diagnosis of B.henselae infections. However different genotype strains and cross reactions with other infectious agents should be investigated by further studies before routine applications of HeLa cell co-cultivations procedure is established.
亨氏巴尔通体感染的实验室诊断主要基于间接免疫荧光法（IFA）的血清学检测。与亨氏巴尔通体进行细胞系共培养以及使用琼脂衍生抗原是评估抗巴尔通体抗体的两种主要方法。Vero细胞系和Hep-2细胞系是内部和商业诊断试剂盒生产中最常用的共培养培养基。然而，易于获取和培养的HeLa细胞也很容易被亨氏巴尔通体感染。本研究的目的是比较在IFA血清学中，通过将亨氏巴尔通体ATCC 49882（休斯顿-1）分别与Vero细胞和HeLa细胞共培养所获得的抗原的性能。在381份血清样本中，两种细胞系共培养进行IFA检测时，127份（33.3%）呈阳性，195份（51.2%）呈阴性。两种方法的总体一致性为84.5%（322/381），计算得出的科恩kappa值为0.68（强，一致）。结果发现，HeLa细胞可用于制备用于IFA检测以进行亨氏巴尔通体感染血清学诊断的亨氏巴尔通体抗原。然而，在建立HeLa细胞共培养程序的常规应用之前，应通过进一步研究调查不同基因型菌株以及与其他感染因子的交叉反应。
