Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
Bioresour Technol. 2012 Apr;109:304-7. doi: 10.1016/j.biortech.2011.07.090. Epub 2011 Aug 3.
A laboratory study was conducted to evaluate the possibility of using wastewater from a soybean curd manufacturing plant as a growth promoter of Botryococcus braunii strain BOT-22. Soybean curd wastewater (SCW) were added to AF-6 medium to set the final concentration to 0% (control), 1%, 2%, 5%, and 10% (v/v). The growth and hydrocarbon production observed in the cultures with 1% and 2% SCW were significantly higher than that observed in the control. It was postulated that proteins and/or reducing sugars in SCW could enhance the growth. A major finding was a shift in the chemical composition of hydrocarbons from C(34)H(58) to C(32)H(54) in association with increased concentrations of SCW. Considering the inorganic ions in SCW, it was presumed that a mixture of nitrate, 1-2% SCW, and secondarily treated SCW can be applied for mass cultivation of Botryococcus.
进行了一项实验室研究，以评估将豆腐制造工厂的废水用作 Botryococcus braunii 菌株 BOT-22 的生长促进剂的可能性。将豆腐废水 (SCW) 添加到 AF-6 培养基中，最终浓度分别设置为 0%（对照）、1%、2%、5%和 10%（v/v）。在含有 1%和 2% SCW 的培养物中观察到的生长和烃类生产明显高于对照。据推测，SCW 中的蛋白质和/或还原糖可以促进生长。一个主要发现是与 SCW 浓度增加相关的碳氢化合物的化学组成从 C(34)H(58)转变为 C(32)H(54)。考虑到 SCW 中的无机离子，可以假定可以将硝酸盐、1-2% SCW 和经过二次处理的 SCW 的混合物用于 Botryococcus 的大规模培养。
