Department of Medicine II, SLK-Kliniken Heilbronn, Academic Hospital of the University of Heidelberg, Heidelberg, Germany.
Adv Med Sci. 2011;56(2):138-44. doi: 10.2478/v10039-011-0026-y.
Although a wide range of biliary plastic and metal stents is on offer nowadays, the ideal cost-effective stent that functions permanently and that is easy to handle regarding its exchange is still not available. Therefore we tested in an in vitro model if the coating of plastic stents with hydrophobin alone or with hydrophobin and antibiotics or heparin in combination leads to an inhibition of the clogging process.
We coated commercially available biliary plastic stents with hydrophobin alone, as well as with hydrophobin and antibiotics or heparin in combination. After an incubation period of 28 days in human bile, we examined the stents by scanning electron microscopy to see whether the clogging material on its surface was reduced.
Coating of plastic stents with hydrophobin led to a reduction in the amount of adherent material on the surface of the stents. Coupling of ampicillin/sulbactam or levofloxacin did not lead to a further reduction of the clogging material, whereas coupling with highly concentrated heparin did reduce the adherent material.
The coating of biliary plastic stents with hydrophobin or with hydrophobin and heparin in combination seems to be a promising option to delay the clogging process.
尽管目前提供了广泛的胆道塑料和金属支架，但仍然没有一种理想的具有成本效益的、能永久发挥作用且易于更换的支架。因此，我们在体外模型中测试了单独用疏水蛋白或与抗生素或肝素组合用疏水蛋白对塑料支架进行涂层是否会抑制堵塞过程。
我们用疏水蛋白单独对市售胆道塑料支架进行涂层，以及与抗生素或肝素组合进行涂层。在人类胆汁中孵育 28 天后，我们通过扫描电子显微镜检查支架，以观察其表面的堵塞材料是否减少。
用疏水蛋白对塑料支架进行涂层可减少支架表面附着的材料数量。将氨苄西林/舒巴坦或左氧氟沙星偶联并不会进一步减少堵塞材料，而将高浓度肝素偶联则会减少附着的材料。
用疏水蛋白或与肝素组合用疏水蛋白对胆道塑料支架进行涂层似乎是延迟堵塞过程的一种有前途的选择。
