Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.
RNA Biol. 2011 Nov-Dec;8(6):978-83. doi: 10.4161/rna.8.6.17907. Epub 2011 Nov 1.
Post-transcriptional regulation of gene expression is ubiquitous and fundamental for the control of cell growth, differentiation and the complex developmental programs of multicellular eukaryotes. Despite this realization, the current tools that are available to study RNAs are limited in many respects. Recently we expanded the RNA recognition code of Pumilio and FBF homology (PUF) proteins, enabling RNA-binding proteins with programmable specificities to be designed. The design of proteins that can bind any RNA sequence of interest and modulate its function will be important to elucidate the mechanisms by which gene expression is controlled at the level of RNA and may provide potential therapeutics in the future.
基因表达的转录后调控普遍存在，对于控制细胞生长、分化和多细胞真核生物的复杂发育程序至关重要。尽管已经认识到这一点，但目前可用于研究 RNA 的工具在许多方面都受到限制。最近，我们扩展了 Pumilio 和 FBF 同源（PUF）蛋白的 RNA 识别密码，从而能够设计出具有可编程特异性的 RNA 结合蛋白。设计能够结合任何感兴趣的 RNA 序列并调节其功能的蛋白质对于阐明基因表达在 RNA 水平上受到控制的机制将非常重要，并可能为未来提供潜在的治疗方法。
