Kaminski Catherine N, Ferrey Seth L, Lowrey Timothy, Guerra Leo, VAN Slambrouck Severine, Steelant Wim F A
Laboratory of Biochemical and Biomedical Research, Department of Chemistry, New Mexico Tech, Socorro.
Oncol Lett. 2010 Jul;1(4):711-715. doi: 10.3892/ol_00000124.
Three different extract conditions (aqueous, EtOH and EtOAc) of four different parts (bracts, leaves, roots and stems) of the plant Anemopsis californica (A. californica) were evaluated for their effect on the growth and migration of human colon cancer cells, HCT-8, and the breast cancer cell lines Hs 578T and MCF-7/AZ. Our aim was to identify potential anticancer activity in crude A. californica extracts, given that this plant is used by Native Americans to treat a variety of diseases, including cancer. Our results demonstrated that for each of the cell lines tested, the majority of ethyl acetate extracts of all the plant parts are more toxic than the aqueous and ethanol extracts. Furthermore, significant growth inhibitory activity against the three cell lines was found for the ethyl acetate extract of the roots, while the aqueous extract of the roots influenced the migratory capacity of the three cell lines. This study provides evidence for the anticancer properties of A. californica when extracted in water and ethyl acetate, and supports the importance for further purification of the crude extracts and isolation of potential new anticancer compounds through bio-guided fractionation.
对加州银线草（Anemopsis californica）四个不同部位（苞片、叶子、根和茎）的三种不同提取条件（水提、乙醇提取和乙酸乙酯提取）进行了评估，以研究其对人结肠癌细胞HCT - 8以及乳腺癌细胞系Hs 578T和MCF - 7/AZ生长和迁移的影响。鉴于这种植物被美洲原住民用于治疗包括癌症在内的多种疾病，我们的目的是确定加州银线草粗提物中潜在的抗癌活性。我们的结果表明，对于所测试的每种细胞系，所有植物部位的大多数乙酸乙酯提取物比水提物和乙醇提取物毒性更大。此外，发现根部的乙酸乙酯提取物对这三种细胞系具有显著的生长抑制活性，而根部的水提物影响了这三种细胞系的迁移能力。本研究为用水和乙酸乙酯提取的加州银线草的抗癌特性提供了证据，并支持了通过生物导向分级进一步纯化粗提物和分离潜在新抗癌化合物的重要性。
