Lee Sang Jun, Lee Ki Won, Hur Haeng Jeon, Chun Ji Young, Kim Seo Young, Lee Hyong Joo
School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Korea.
Ann N Y Acad Sci. 2007 Jan;1095:536-44. doi: 10.1196/annals.1397.058.
Considerable attention has recently been focused on identifying chemopreventive phytochemicals derived from medicinal plants. Here, we analyzed phenolic phytochemicals from red pine (RP) leaves and found epigallocatechin gallate (EGCG) and epigallocatechin (EGC), and catechin gallate (CG) as their major phenolic phytochemicals. This article also investigated whether RP leaf extract and its phenolic phytochemicals inhibit the invasion of SK-Hep-1 human hepatocellular carcinoma cells (SK-Hep-1 cells). RP suppressed the invasion and the migration of SK-Hep-1 cells. EGCG and CG also inhibited the invasion and migration, with EGC exhibiting a lower efficacy. Matrix metalloproteinases (MMPs), particularly gelatinase-A (MMP-2) and gelatinase-B (MMP-9), degrade components of the basement membrane and are strongly implicated in invasion and metastasis formation of malignant tumors. RP suppressed both MMP-2 and MMP-9 activities. EGCG and CG reduced the activities of MMP-9 and MMP-2 in a dose-dependent manner, with EGC exhibiting a lower efficacy on both MMPs. Our results suggest that RP inhibits tumor invasion and migration, which may be attributed to the effects of EGCG and CG. In particular, EGCG plays a key role in the efficacy of RP against hepatocarcinogenesis.
近来，相当多的注意力集中在鉴定源自药用植物的化学预防植物化学物质上。在此，我们分析了红松（RP）叶中的酚类植物化学物质，发现表没食子儿茶素没食子酸酯（EGCG）、表没食子儿茶素（EGC）和儿茶素没食子酸酯（CG）是其主要的酚类植物化学物质。本文还研究了RP叶提取物及其酚类植物化学物质是否抑制SK - Hep - 1人肝癌细胞（SK - Hep - 1细胞）的侵袭。RP抑制了SK - Hep - 1细胞的侵袭和迁移。EGCG和CG也抑制了侵袭和迁移，而EGC的功效较低。基质金属蛋白酶（MMPs），特别是明胶酶 - A（MMP - 2）和明胶酶 - B（MMP - 9），可降解基底膜成分，并与恶性肿瘤的侵袭和转移形成密切相关。RP抑制了MMP - 2和MMP - 9的活性。EGCG和CG以剂量依赖的方式降低了MMP - 9和MMP - 2的活性，EGC对两种MMPs的功效较低。我们的结果表明，RP抑制肿瘤侵袭和迁移，这可能归因于EGCG和CG的作用。特别是，EGCG在RP抗肝癌发生的功效中起关键作用。
