Grandfils C, Bettendorff L, de Rycker C, Schoffeniels E
Laboratoire de Biochimie Générale et Comparée, Université de Liège, Belgium.
Anal Biochem. 1988 Mar;169(2):274-8. doi: 10.1016/0003-2697(88)90285-0.
We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
