Department of Medical Affairs, Mead Johnson Nutrition, Evansville, IN, USA.
J Pediatr Gastroenterol Nutr. 2012 Mar;54(3):343-52. doi: 10.1097/MPG.0b013e318237ed95.
The aim of the study was to evaluate the effect of infant formula with polydextrose (PDX) and galacto-oligosaccharides (GOS) on fecal microbiota and secretory IgA (sIgA).
In the present double-blind, randomized study, term infants received control (Enfamil Lipil) or the same formula with PDX/GOS (4 g/L, 1:1 ratio; PDX/GOS) for 60 days; a reference breast-fed group was included. Formula intake, tolerance, and stool characteristics were collected via electronic diary and analyzed by repeated measures analysis of variance. Anthropometric measurements and stool samples were obtained at baseline and after 30 and 60 days of feeding. Fecal sIgA was measured by enzyme-linked immunosorbent assay and fecal bacteria by fluorescent in situ hybridization and quantitative real-time polymerase chain reaction (qPCR); both were analyzed by Wilcoxon rank sum test.
Two hundred thirty infants completed the study. Infants consuming PDX/GOS had softer stools than control at all times (P < 0.001). Using qPCR, counts in PDX/GOS were closer to the breast-fed group, tended to be higher than control for total bifidobacteria (P = 0.069) and Bifidobacterium longum (P = 0.057) at 30 days, and were significantly higher for total bifidobacteria and B longum at 60 days and B infantis at 30 days (P = 0.002). No significant differences were detected between PDX/GOS and control in changes from baseline to 30 or 60 days for sIgA or total bifidobacteria by fluorescent in situ hybridization or qPCR; however, significantly higher changes from baseline were detected between PDX/GOS and control for B infantis at 30 days and B longum at 60 days (P ≤ 0.035).
Infant formula with PDX/GOS produces soft stools and a bifidogenic effect closer to breast milk than formula without PDX/GOS.
本研究旨在评估含有聚葡萄糖（PDX）和半乳糖寡糖（GOS）的婴儿配方奶粉对粪便微生物群和分泌型免疫球蛋白 A（sIgA）的影响。
在这项双盲、随机研究中，足月婴儿接受对照（Enfamil Lipil）或相同配方的 PDX/GOS（4 g/L，1:1 比例；PDX/GOS）60 天；纳入了一个参考母乳喂养组。通过电子日记收集配方摄入量、耐受性和粪便特征，并通过重复测量方差分析进行分析。在基线和喂养 30 天和 60 天后进行人体测量测量和粪便样本采集。通过酶联免疫吸附测定法测量粪便 sIgA，通过荧光原位杂交和定量实时聚合酶链反应（qPCR）测量粪便细菌，并通过 Wilcoxon 秩和检验进行分析。
230 名婴儿完成了研究。与对照组相比，摄入 PDX/GOS 的婴儿在所有时间点的粪便均更软（P<0.001）。使用 qPCR，PDX/GOS 的计数更接近母乳喂养组，双歧杆菌总数（P=0.069）和长双歧杆菌（P=0.057）在 30 天时倾向于高于对照组，双歧杆菌总数和长双歧杆菌在 60 天时以及 B infantis 在 30 天时均显著高于对照组（P=0.002）。在 30 天或 60 天的 sIgA 或荧光原位杂交或 qPCR 中，PDX/GOS 和对照组之间没有检测到从基线到 30 天或 60 天的变化有显著差异；然而，在 30 天时，PDX/GOS 和对照组之间的 B infantis 和 60 天时的 B longum 的变化有显著差异（P≤0.035）。
与不含 PDX/GOS 的配方相比，含有 PDX/GOS 的婴儿配方可产生更软的粪便，并产生更接近母乳的双歧杆菌效应。
