Zhu Liying, Guo Fangjie, Guo Zeyu, Chen Xiaoqiang, Qian Xiaoguo, Li Xianglong, Li Xiaoqiong, Li Jinjun, Wang Xin, Jia Weiguo
Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Henan Tailijie Biotech Co., Ltd., Mengzhou, China.
Front Nutr. 2022 Jul 29;9:934621. doi: 10.3389/fnut.2022.934621. eCollection 2022.
Polydextrose is a nutrient supplement, which is widely applied in the food industry. The use of polydextrose in combination with prebiotics and probiotics has recently increased, whereas the fermentation properties of its blend have not yet been fully revealed. We evaluated the metabolic profile of polydextrose, inulin, and their blends by a batch fermentation of fifteen human fecal inocula. After 24 h of fermentation, polydextrose increased the production of gas, ammonia, and several short chain fatty acids, including propionate and butyrate, when compared to its blends, inulin, and fructo-oligosaccharides. Furthermore, polydextrose had the slowest degradation rate of all the carbohydrates tested, consistent with its partial fermentation in the distal colon. The 16S rRNA gene sequencing analysis of the gut microbiome exhibited significantly increased relative abundance of , , , and in polydextrose compared to other carbohydrates. On the other hand, the blends of polydextrose and inulin (1:1 or 2:1) showed reduced gas production and similar bifidogenicity to inulin alone. The blends not only had similar alpha-diversity and PCoA to inulin but also had a similar abundance of beneficial bacteria, such as and , suggesting potential health benefits. Also their low gas production was likely due to the abundance of and , which were negatively correlated with gas production. Additionally, our fermentation model shows advantages in the large-scale assessment of fermentation performance.
聚葡萄糖是一种营养补充剂，广泛应用于食品工业。聚葡萄糖与益生元和益生菌联合使用的情况近来有所增加，但其混合物的发酵特性尚未完全揭示。我们通过对15份人类粪便接种物进行分批发酵，评估了聚葡萄糖、菊粉及其混合物的代谢概况。发酵24小时后，与聚葡萄糖混合物、菊粉和低聚果糖相比，聚葡萄糖增加了气体、氨和几种短链脂肪酸（包括丙酸和丁酸）的产生。此外，聚葡萄糖在所有测试碳水化合物中降解速率最慢，这与其在结肠远端的部分发酵一致。肠道微生物群的16S rRNA基因测序分析显示，与其他碳水化合物相比，聚葡萄糖中 、 、 和 的相对丰度显著增加。另一方面，聚葡萄糖和菊粉的混合物（1:1或2:1）产气减少，双歧杆菌生成能力与单独使用菊粉相似。这些混合物不仅与菊粉具有相似的α多样性和主坐标分析，而且有益细菌（如 和 ）的丰度也相似，表明具有潜在的健康益处。它们产气少可能是由于 和 含量丰富，这两种菌与产气呈负相关。此外，我们的发酵模型在大规模评估发酵性能方面具有优势。
