Jin Y, Yang L J
Department of Oral Pathology, Stomatological College, Fourth Military Medical University, Xian, China.
J Oral Pathol Med. 1990 Apr;19(4):152-4. doi: 10.1111/j.1600-0714.1990.tb00815.x.
The monoclonal antibody against bovine bone morphogenetic protein (bBMP-McAb) was first used for demonstration of bone morphogenetic protein (BMP) in osteosarcoma. The avidin-biotin complex method (ABC) demonstrated that of the 18 osteosarcomas, 15 stained positive, while all 6 fibrosarcomas were negative. The results showed that BMP mainly exists in the tumor cell plasma and some tumor-like bone tissues. Using this staining method, we can not only differentiate osteosarcoma from fibrosarcoma and other non-bone-derived tumors, but also classify osteosarcoma according to the content and distribution of BMP and the patient's clinical situation, thus providing a scientific basis for clinical treatment.
抗牛骨形态发生蛋白单克隆抗体（bBMP-McAb）首次用于骨肉瘤中骨形态发生蛋白（BMP）的检测。抗生物素蛋白-生物素复合物法（ABC法）显示，18例骨肉瘤中有15例呈阳性染色，而6例纤维肉瘤均为阴性。结果表明，BMP主要存在于肿瘤细胞质及部分肿瘤样骨组织中。应用该染色方法，不仅可以鉴别骨肉瘤与纤维肉瘤及其他非骨源性肿瘤，还能根据BMP的含量、分布及患者的临床情况对骨肉瘤进行分类，从而为临床治疗提供科学依据。
