Applied Materials Science, Department of Engineering Sciences, Uppsala University, Uppsala, Sweden.
J Biomed Mater Res B Appl Biomater. 2012 Jan;100(1):82-93. doi: 10.1002/jbm.b.31925. Epub 2011 Sep 27.
A bioactive mesoporous titanium dioxide (MT) coating for surface drug delivery has been investigated to develop a multifunctional implant coating, offering quick bone bonding and biological stability. An evaporation induced self-assembly (EISA) method was used to prepare a mesoporous titanium dioxide coating of the anatase phase with BET surface area of 172 m(2)/g and average pore diameter of 4.3 nm. Adhesion tests using the scratch method and an in situ screw-in/screw-out technique confirm that the MT coating bonds tightly with the metallic substrate, even after removal from bone. Because of its high surface area, the bioactivity of the MT coating is much better than that of a dense TiO(2) coating of the same composition. Quick formation of hydroxyapatite (HA) in vitro can be related to enhance bonding with bone. The uptake of antibiotics by the MT coating reached 13.4 mg/cm(3) within a 24 h loading process. A sustained release behavior has been obtained with a weak initial burst. By using Cephalothin as a model drug, drug loaded MT coating exhibits a sufficient antibacterial effect on the material surface, and within millimeters from material surface, against E.coli. Additionally, the coated and drug loaded surfaces showed no cytotoxic effect on cell cultures of the osteoblastic cell line MG-63. In conclusion, this study describes a novel, biocompatiblemesoporous implant coating, which has the ability to induce HA formation and could be used as a surface drug-delivery system.
一种用于表面药物输送的生物活性介孔二氧化钛 (MT) 涂层已被研究开发出一种多功能植入物涂层，提供快速的骨结合和生物稳定性。蒸发诱导自组装 (EISA) 方法用于制备具有 BET 表面积为 172 m(2)/g 和平均孔径为 4.3nm 的锐钛矿相介孔二氧化钛涂层。使用划痕法和原位旋入/旋出技术进行的附着力测试证实，即使从骨头上取下，MT 涂层也与金属基底紧密结合。由于其高表面积，MT 涂层的生物活性远优于相同组成的致密 TiO(2)涂层。体外快速形成羟基磷灰石 (HA) 可归因于增强与骨的结合。MT 涂层在 24 小时加载过程中达到 13.4mg/cm(3)的抗生素摄取量。通过使用头孢菌素作为模型药物，负载药物的 MT 涂层在材料表面上表现出足够的抗菌效果，并且在材料表面几毫米范围内对大肠杆菌具有抗菌效果。此外，涂覆和负载药物的表面对成骨细胞系 MG-63 的细胞培养物没有细胞毒性作用。总之，本研究描述了一种新型的生物相容的介孔植入物涂层，它具有诱导 HA 形成的能力，并可用作表面药物输送系统。
