Liang Haidong, Yu Fang, Tong Zhihong, Huang Zaiguo
Hands and feet microsurgery, Dalian Municipal Central Hospital, Dalian 116033, China; E-Mail:
Int J Mol Sci. 2011;12(8):5060-9. doi: 10.3390/ijms12085060. Epub 2011 Aug 8.
To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg(-1), i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, laparotomized, and sutured without removing their ovaries. After 1 week of recovery from surgery, the OVX rats were randomly divided into three groups and orally treated with H(2)O (OVX group) or CHE (100 or 200 mg kg(-1) daily) for 3 months. The sham-operated group (n = 8) was orally treated with H(2)O. After 3 months, the total body bone mineral density (BMD), bone mineral content (BMC), Bone biomechanical index, blood mineral levels and blood antioxidant enzymes activities were examined in sham-operated, ovariectomized and Cistanches Herba extract treated rats. Results showed that Cistanches Herba extract treatment significantly dose-dependently enhanced bone mineral density (BMD), bone mineral content (BMC), maximum load, displacement at maximum load, stress at maximum load, load at auto break, displacement at auto break, and stress at auto break, and blood antioxidant enzymes activities, decreased blood Ca, Zn and Cu levels compared to the OVX group. This experiment demonstrates that the administration of Cistanches Herba extract to ovariectomized rats reverses bone loss and prevents osteoporosis.
为评估中药肉苁蓉提取物（CHE）预防去卵巢（OVX）大鼠骨质流失的能力，将肉苁蓉提取物（CHE）经胃内给予大鼠。雌性大鼠用戊巴比妥钠（40 mg kg⁻¹，腹腔注射）麻醉，双侧卵巢切除。假手术组大鼠麻醉后剖腹并缝合，不切除卵巢。术后恢复1周后，将去卵巢大鼠随机分为三组，分别口服给予水（OVX组）或CHE（每日100或200 mg kg⁻¹），持续3个月。假手术组（n = 8）口服给予水。3个月后，检测假手术组、去卵巢组和肉苁蓉提取物处理组大鼠的全身骨矿物质密度（BMD）、骨矿物质含量（BMC）、骨生物力学指标、血液矿物质水平和血液抗氧化酶活性。结果显示，与OVX组相比，肉苁蓉提取物处理显著剂量依赖性地提高了骨矿物质密度（BMD）、骨矿物质含量（BMC）、最大负荷、最大负荷时的位移、最大负荷时的应力、自动断裂时的负荷、自动断裂时的位移和自动断裂时的应力，以及血液抗氧化酶活性，降低了血液中钙、锌和铜的水平。本实验表明，给去卵巢大鼠施用肉苁蓉提取物可逆转骨质流失并预防骨质疏松症。
