Saravanakumar Kandasamy, Park SeonJu, Sathiyaseelan Anbazhagan, Kim Kil-Nam, Cho Su-Hyeon, Mariadoss Arokia Vijaya Anand, Wang Myeong-Hyeon
Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Korea.
Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
Pharmaceuticals (Basel). 2021 Jan 28;14(2):102. doi: 10.3390/ph14020102.
In this study, the methanolic extract from seeds of exhibited strong antioxidant and enzyme inhibition activities with less toxicity to NIH3T3 and HepG2 cells at the concentration of 100 µg/mL. The antioxidant activities (DPPH and ABTS), α-amylase, and α-glucosidase inhibition activities were found higher in methanolic extract (MeOH-E) than HO extract. Besides, 9.82 ± 0.62 µg and 6.42 ± 0.26 µg of MeOH-E were equivalent to 1 µg ascorbic acid for ABTS and DPPH scavenging, respectively while 9.02 ± 0.25 µg and 6.52 ± 0.15 µg of MeOH-E were equivalent to 1 µg of acarbose for inhibition of α-amylase and α-glucosidase respectively. Moreover, the cell assay revealed that the addition of MeOH-E (12.5 µg/mL) increased about 37% of glucose uptake in insulin resistant (IR) HepG2 as compared to untreated IR HepG2 cells. The LC- MS/MS and GC-MS analysis of MeOH-E revealed a total of 54 compounds including terpenoids, glycosides, fatty acid, phenolic acid derivatives. Among the identified compounds, chlorogenic acid and jasminoside A were found promising for anti-diabetic activity revealed by molecular docking study and these molecules are deserving further purification and molecular analysis.
在本研究中，[植物名称]种子的甲醇提取物在100 µg/mL浓度下表现出较强的抗氧化和酶抑制活性，对NIH3T3和HepG2细胞的毒性较小。发现甲醇提取物（MeOH-E）的抗氧化活性（DPPH和ABTS）、α-淀粉酶和α-葡萄糖苷酶抑制活性高于水提取物（HO提取物）。此外，对于ABTS和DPPH清除，9.82±0.62 µg和6.42±0.26 µg的MeOH-E分别相当于1 µg抗坏血酸，而对于α-淀粉酶和α-葡萄糖苷酶的抑制，9.02±0.25 µg和6.52±0.15 µg的MeOH-E分别相当于1 µg阿卡波糖。此外，细胞试验表明，与未处理的胰岛素抵抗（IR）HepG2细胞相比，添加MeOH-E（12.5 µg/mL）可使IR HepG2细胞的葡萄糖摄取增加约37%。MeOH-E的LC-MS/MS和GC-MS分析共鉴定出54种化合物，包括萜类、糖苷、脂肪酸、酚酸衍生物。在鉴定出的化合物中，通过分子对接研究发现绿原酸和茉莉糖苷A具有抗糖尿病活性，这些分子值得进一步纯化和分子分析。
