Xiao Xin-Yu, Cui Long-Hai, Zhou Xin-Xin, Wu Yan, Ge Fa-Huan
Guangzhou University of TCM, Guangzhou 510405, China.
Zhong Yao Cai. 2011 May;34(5):789-94.
The orthogonal test and the supercritical carbon dioxide fluid extraction were used for optimizing the extraction of the essential oil from Plumeria rubra var. actifolia for the first time. Compared with the steam distillation, the optimal operation parameter of extraction was as follows: extraction pressure 25 MPa, extraction temperature 45 degrees C; separator I pressure 12 MPa, separator I temperature 55 degrees C; separator II pressure 6 MPa, separator II temperature 30 degrees C. Under this condition the yield of the essential oil was 5.8927%. The components were separated and identified by GC-MS. 53 components of Plumeria rubra var. actifolia measured by SFE method were identified and determined by normalization method. The main components were 1, 6, 10-dodecatrien-3-ol, 3, 7, 11-trimethyl, benzoic acid, 2-hydroxy-, phenylmethyl ester, 1, 2-benzenedicarboxylic acid, bis(2-methylpropyl) ester,etc.. 1, 2-Benzenedicarboxylic acid, bis (2-methylpropyl) este. took up 66.11% of the total amount, and there was much difference of the results from SD method.
首次采用正交试验和超临界二氧化碳流体萃取法对鸡蛋花精油的提取工艺进行优化。与水蒸气蒸馏法相比，最佳萃取操作参数如下：萃取压力25MPa，萃取温度45℃；一级分离器压力12MPa，一级分离器温度55℃；二级分离器压力6MPa，二级分离器温度30℃。在此条件下，精油得率为5.8927%。采用气相色谱-质谱联用仪（GC-MS）对其成分进行分离鉴定，用归一化法对超临界流体萃取法测定的鸡蛋花53种成分进行了鉴定和定量分析。主要成分有1,6,10-十二碳三烯-3-醇，3,7,11-三甲基，苯甲酸，2-羟基-，苯甲酯，1,2-苯二甲酸，双（2-甲基丙基）酯等。1,2-苯二甲酸，双（2-甲基丙基）酯占总量的66.11%，与水蒸气蒸馏法的结果有较大差异。
