Kiyamova R, Shyian M, Lyzogubov V V, Usenko V S, Gout T, Filonenko V
Department of Cell Signaling, Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv, 03680, Ukraine.
Exp Oncol. 2011 Sep;33(3):157-61.
To study the expression profile of the NaPi2b protein and its localization in breast, ovarian and lung cancer cells in relation to normal tissues adjacent to tumor.
Immunohistochemical analysis with monoclonal antibody MX35 was applied for investigation of NaPi2b protein expression in breast, lung and ovarian carcinomas. Intensity of NaPi2b protein expression was calculated with semiquantitative scores.
NaPi2b (MX35) protein expression was detected in breast, lung and ovarian cancer cells and adjacent normal tissue. We have shown that in contrast to ovarian tumors in breast and lung tumors NaPi2b expression is down regulated comparing to correspondent normal tissues.
This study provides the data on the pattern of NaPi2b expression and cellular localization in breast, lung and ovarian cancers, which might be useful for understanding the mechanism of transport and maintenance of inorganic phosphate in cancer and normal cells, as well as for developing novel immunotherapeutic approaches based on MX35 monoclonal antibody.
研究NaPi2b蛋白在乳腺癌、卵巢癌和肺癌细胞中的表达谱及其与肿瘤旁正常组织相比的定位情况。
应用单克隆抗体MX35进行免疫组化分析，以研究NaPi2b蛋白在乳腺癌、肺癌和卵巢癌中的表达情况。通过半定量评分计算NaPi2b蛋白表达强度。
在乳腺癌、肺癌和卵巢癌细胞以及相邻正常组织中均检测到NaPi2b（MX35）蛋白表达。我们发现，与卵巢肿瘤不同，乳腺癌和肺癌中的NaPi2b表达与相应正常组织相比下调。
本研究提供了关于NaPi2b在乳腺癌、肺癌和卵巢癌中的表达模式及细胞定位的数据，这可能有助于理解癌症和正常细胞中无机磷酸盐的转运和维持机制，以及基于MX35单克隆抗体开发新的免疫治疗方法。
