接种层出镰刀菌和尖孢镰刀菌后芦笋中的自由基、水杨酸和霉菌毒素

Free Radicals, Salicylic Acid and Mycotoxins in Asparagus After Inoculation with Fusarium proliferatum and F. oxysporum.

作者信息

Dobosz Bernadeta, Drzewiecka Kinga, Waskiewicz Agnieszka, Irzykowska Lidia, Bocianowski Jan, Karolewski Zbigniew, Kostecki Marian, Kruczynski Zdzislaw, Krzyminiewski Ryszard, Weber Zbigniew, Golinski Piotr

出版信息

Appl Magn Reson. 2011 Sep;41(1):19-30. doi: 10.1007/s00723-011-0233-5. Epub 2011 Jun 9.

DOI:10.1007/s00723-011-0233-5

PMID:21957331

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3162185/

Abstract

Electron paramagnetic resonance was used to monitor free radicals and paramagnetic species like Fe, Mn, Cu generation, stability and status in Asparagus officinalis infected by common pathogens Fusarium proliferatum and F. oxysporum. Occurrence of F. proliferatum and F. oxysporum, level of free radicals and other paramagnetic species, as well as salicylic acid and mycotoxins content in roots and stems of seedlings were estimated on the second and fourth week after inoculation. In the first term free and total salicylic acid contents were related to free radicals level in stem (P = 0.010 and P = 0.033, respectively). Concentration of Fe(3+) ions in porphyrin complexes (g = 2.3, g = 2.9) was related to the species of pathogen. There was no significant difference between Mn(2+) concentrations in stem samples; however, the level of free radicals in samples inoculated with F. proliferatum was significantly higher when compared to F. oxysporum.

摘要

利用电子顺磁共振技术监测了受常见病原菌轮枝镰孢菌（Fusarium proliferatum）和尖孢镰刀菌（F. oxysporum）侵染的芦笋（Asparagus officinalis）中自由基以及铁、锰、铜等顺磁物质的生成、稳定性和状态。在接种后的第二周和第四周，对幼苗根和茎中轮枝镰孢菌和尖孢镰刀菌的发生情况、自由基及其他顺磁物质的水平，以及水杨酸和霉菌毒素的含量进行了评估。在第一阶段，游离水杨酸和总水杨酸含量与茎中的自由基水平相关（P分别为0.010和0.033）。卟啉配合物中Fe(3+)离子的浓度（g = 2.3，g = 2.9）与病原菌种类有关。茎样品中Mn(2+)的浓度之间没有显著差异；然而，与接种尖孢镰刀菌的样品相比，接种轮枝镰孢菌的样品中的自由基水平显著更高。