Cellulosic Ethanol Program, Institute of Nuclear Energy Research, Taoyuan, Taiwan, ROC.
Bioresour Technol. 2011 Nov;102(22):10451-8. doi: 10.1016/j.biortech.2011.08.118. Epub 2011 Sep 10.
A combination of a twin-screw extrusion and an acid-catalyzed hot water extraction process performed at a bench-scale was used to prepare high monomeric xylose hydrolysate for cellulosic production. The influences of the screw speed (30-150 rpm), barrel temperature (80-160 °C) and corresponding specific mechanical energy of the extruder on the structural properties of the pretreated rice straw, sugar concentration and conversion were investigated. The optimal condition for the extrusion step was determined to be 40 rpm with 3% H2SO4 at 120 °C; the optimal condition for the extraction step was determined to be 130 °C for 20 min. After the pretreatment at the optimal condition, 83.7% of the xylan was converted to monomeric xylose, and the concentration reached levels of 53.7 g/L. Finally, after the subsequent enzymatic hydrolysis, an 80% yield of the total saccharification was obtained.
采用双螺杆挤压与酸催化热水提取相结合的方法，在中试规模下制备了用于纤维素生产的高单体木糖水解液。考察了螺杆转速（30-150 rpm）、机筒温度（80-160°C）和相应的挤出机比机械能对预处理稻草结构性能、糖浓度和转化率的影响。确定挤压步骤的最佳条件为 40 rpm 下添加 3%硫酸在 120°C；提取步骤的最佳条件为 130°C 提取 20 min。在最佳条件下预处理后，83.7%的木聚糖转化为单体木糖，浓度达到 53.7 g/L。最后，经过后续的酶解，总糖化率达到 80%。
