College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
Fitoterapia. 2011 Dec;82(8):1295-301. doi: 10.1016/j.fitote.2011.09.003. Epub 2011 Sep 19.
Pogostemonis Herba has long been used in traditional Chinese medicine for the treatment of inflammatory disorders. Patchouli alcohol (PA), a tricyclic sesquiterpene isolated from Pogostemonis Herba, is known to possess a variety of pharmacological activities. The present study aimed to investigate the in vivo anti-inflammatory effect of PA using two common inflammatory animal models i.e., xylene-induced ear edema in mice and carrageenan-induced paw edema in rats. The degree of edema in both inflammatory animals, as well as the protein and mRNA expression of some inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), prostaglandin E₂ (PGE₂) and nitric oxide (NO) in the hind paw of carrageenan-treated rats were measured. Results showed that PA (10-40 mg/kg) significantly inhibited the ear edema induced by xylene in mice and the paw edema induced by carrageenan in rats. In addition, treatment with PA (10-40 mg/kg) also dose-dependently decreased the production of TNF-α, IL-1β, PGE₂ and NO in the hind paw of carrageenan-treated rats. Furthermore, PA treatment also suppressed the mRNA expression of TNF-α, IL-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the hind paw of carrageenan-treated rats. These results suggest that PA possesses potent anti-inflammatory activity, which may be mediated, at least in part, by down-regulating the mRNA expression of a panel of inflammatory mediators including TNF-α, IL-1β, iNOS and COX-2.
佩兰（ Pogostemonis Herba ）在传统中医中已被用于治疗炎症性疾病。百秋李醇（ PA ）是从佩兰（ Pogostemonis Herba ）中分离出来的三环倍半萜烯，已知具有多种药理活性。本研究旨在通过两种常见的炎症动物模型，即二甲苯诱导的小鼠耳肿胀和角叉菜胶诱导的大鼠足肿胀，研究 PA 的体内抗炎作用。测量了两种炎症动物的肿胀程度，以及角叉菜胶处理大鼠后爪中一些炎症介质（包括肿瘤坏死因子-α（ TNF-α ）、白细胞介素-1β（ IL-1β ）、前列腺素 E₂（ PGE₂ ）和一氧化氮（ NO ））的蛋白和 mRNA 表达。结果表明， PA （ 10-40mg/kg ）显著抑制了二甲苯诱导的小鼠耳肿胀和角叉菜胶诱导的大鼠足肿胀。此外， PA （ 10-40mg/kg ）治疗还剂量依赖性地降低了角叉菜胶处理大鼠后爪中 TNF-α 、 IL-1β 、 PGE₂ 和 NO 的产生。此外， PA 治疗还抑制了角叉菜胶处理大鼠后爪中 TNF-α 、 IL-1β 、诱导型一氧化氮合酶（ iNOS ）和环氧化酶-2 （ COX-2 ）的 mRNA 表达。这些结果表明， PA 具有强大的抗炎活性，其机制可能至少部分通过下调 TNF-α 、 IL-1β 、 iNOS 和 COX-2 等一系列炎症介质的 mRNA 表达来介导。
