从广藿香中分离出的广藿香醇对脂多糖刺激的RAW264.7巨噬细胞的抗炎作用。
Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages.
作者信息
Xian Yan-Fang, Li Yu-Cui, Ip Siu-Po, Lin Zhi-Xiu, Lai Xiao-Ping, Su Zi-Ren
机构信息
School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong SAR;
出版信息
Exp Ther Med. 2011 May;2(3):545-550. doi: 10.3892/etm.2011.233. Epub 2011 Mar 21.
Abstract
Pogostemonis Herba has long been used in traditional Chinese medicine for the treatment of inflammation-related disorders. Patchouli alcohol (PA) isolated from Pogostemonis Herba is a tricyclic sesquiterpene that is known to exert a variety of pharmacological activities. The present study aimed to investigate the anti-inflammatory effect of PA on lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Pre-treatment with PA at concentrations of 10, 20 or 40 μM dose-dependently decreased the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, nitric oxide (NO) and prostaglandin E(2) in LPS-stimulated RAW264.7 cells. In addition, PA treatment also reversed the increased mRNA expression of TNF-α, IL-1β, IL-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 caused by LPS in RAW264.7 cells. These results indicate that PA is an important anti-inflammatory constituent of Pogostemonis Herba and that its anti-inflammatory effect may be mediated, at least in part, by down-regulation of the mRNA expression of a panel of inflammatory mediators, such as TNF-α, IL-1β, IL-6, iNOS and COX-2.
摘要
广藿香长期以来被用于传统中药中治疗炎症相关疾病。从广藿香中分离出的广藿香醇（PA）是一种三环倍半萜，已知具有多种药理活性。本研究旨在探讨PA对脂多糖（LPS）刺激的RAW264.7细胞的抗炎作用。用10、20或40μM浓度的PA预处理可剂量依赖性地降低LPS刺激的RAW264.7细胞中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6、一氧化氮（NO）和前列腺素E2的产生。此外，PA处理还逆转了LPS导致的RAW264.7细胞中TNF-α、IL-1β、IL-6、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2 mRNA表达的增加。这些结果表明，PA是广藿香的一种重要抗炎成分，其抗炎作用可能至少部分是通过下调一组炎症介质如TNF-α、IL-1β、IL-6、iNOS和COX-2的mRNA表达来介导的。
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