Department of Dentistry and Oral Surgery, School of Medicine, Keio University, 35 Shinanomachi, Sinnjuku-ku, Tokyo, Japan.
Arch Oral Biol. 2012 Apr;57(4):364-8. doi: 10.1016/j.archoralbio.2011.09.005. Epub 2011 Sep 28.
To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens.
Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated.
In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration.
These results suggest that 30s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.
研究聚维酮碘（PVP-I）对牙周病致病菌人工双物种生物膜的抗菌活性。
在肉汤培养基中培养牙龈卟啉单胞菌或核梭杆菌，然后将其接种于聚碳酸酯膜（PCM）组织培养插入物上。孵育 72 小时后，将 PVP-I 溶液应用于生物膜，作用时间从 0.5 至 5 分钟不等。加入灭活剂后，取出每个 PCM，将 PCM 上的生物膜进行连续稀释，并接种于血琼脂平板，在厌氧条件下培养 7 天。然后计数活菌。
在双物种生物膜模型中，与单种生物膜相比，核梭杆菌的活菌数增加了约 200 倍。在双物种生物膜中，需要浓度等于或大于 2%的 PVP-I 才能显著减少牙龈卟啉单胞菌和核梭杆菌。当 PVP-I 的接触时间增加到 1 分钟或更长时间时，任何浓度的 PVP-I 的抗菌活性均无差异。
这些结果表明，30 秒应用 2%的 PVP-I 可以有效抑制双物种生物膜中的牙龈卟啉单胞菌和核梭杆菌，这为控制龈下生物膜提供了临床意义。
