Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore 570011, India.
Phytother Res. 2012 Apr;26(4):587-93. doi: 10.1002/ptr.3611. Epub 2011 Sep 30.
The antifatigue effect of bacoside extract (BME) from Bacopa monniera (L.) Wettst. was investigated. Rats were subjected to weight-loaded forced swim test (WFST) every alternate day for 3 weeks. The BME at a dosage of 10 mg/kg body weight was administered orally to rats for 2 weeks in order to evaluate the following biomarkers of physical fatigue: swimming time, change in body weight, lipid peroxidation, lactic acid (LA), glycogen, antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) and blood parameters, namely blood urea nitrogen (BUN) and creatine kinase (CK). The exhaustive swimming time was increased by 3-fold in the BME supplemented group compared with that of the control group on day 13. The BME treatment lowered malondialdehyde (MDA) levels in brain, liver and muscle tissues by 11.2%, 16.2% and 37.7%, respectively, compared with the control exercised group (p < 0.05). The BME also reduced the LA, serum BUN and CK activities significantly compared with that of the control. Administration of BME significantly protected the depletion of SOD and CAT activities. The HSP-70 expression studies by western blot also confirmed the antifatigue property of BME. The present study thus indicates that BME ameliorates the various impairments associated with physical fatigue.
本研究旨在探讨益智草提取物（BME）的抗疲劳作用。将大鼠每隔一天进行一次负重游泳测试（WFST），共进行 3 周。BME 以 10mg/kg 体重的剂量经口给予大鼠 2 周，以评估以下与体力疲劳相关的生物标志物：游泳时间、体重变化、脂质过氧化、乳酸（LA）、糖原、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等抗氧化酶活性以及血液参数，即血尿素氮（BUN）和肌酸激酶（CK）。与对照组相比，补充 BME 的大鼠在第 13 天的竭性游泳时间增加了 3 倍。与对照组运动组相比，BME 处理分别使大脑、肝脏和肌肉组织中的丙二醛（MDA）水平降低了 11.2%、16.2%和 37.7%（p<0.05）。BME 还显著降低了 LA、血清 BUN 和 CK 活性。Western blot 研究 HSP-70 的表达也证实了 BME 的抗疲劳特性。本研究表明，BME 可改善与体力疲劳相关的各种损伤。
