Xu Meihong, Liang Rui, Li Yong, Wang Junbo
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing, PR China.
Food Nutr Res. 2017 Jun 14;61(1):1334485. doi: 10.1080/16546628.2017.1334485. eCollection 2017.
As the building blocks of nucleic acids, nucleotides are conditionally essential nutrients that exhibit multifaceted activities. The present study aimed to evaluate the anti-fatigue effects of dietary nucleotides (NTs) on mice and explore the possible underlying mechanism. Mice were randomly divided into four experimental sets to detect different indicators. Each set of mice was then divided into four groups: (i) one control group and (ii) three NTs groups, which were fed diets supplemented with NTs at concentrations of 0%, 0.04%, 0.16%, and 0.64% (wt/wt). NTs could significantly increase the forced swimming time, enhance lactate dehydrogenase activity and hepatic glycogen levels, as well as delay the accumulation of blood urea nitrogen and blood lactic acid in mice after 30 days of treatment. NTs also markedly improved fatigue-induced alterations in oxidative stress biomarkers and antioxidant enzymes. Notably, NTs increased the mitochondrial energy metabolic enzyme activities in the skeletal muscles of mice. These results suggest that NTs exert anti-fatigue effects, which may be attributed to the inhibition of oxidative stress and the improvement of mitochondrial function in skeletal muscles. NTs could be used as a novel natural agent for relieving exercise fatigue. : ATP: adenosine triphosphate; BLA: blood lactic acid; GSH-Px: glutathione peroxidase; LDH: lactate dehydrogenase; MDA: malondialdehyde; NTs: dietary nucleotides; SDH: succinate dehydrogenase; SOD: superoxide dismutase; BUN: blood urea nitrogen.
作为核酸的组成成分，核苷酸是具有多方面活性的条件必需营养素。本研究旨在评估膳食核苷酸（NTs）对小鼠的抗疲劳作用，并探索其可能的潜在机制。将小鼠随机分为四组以检测不同指标。然后每组小鼠再分为四组：（i）一个对照组和（ii）三个NTs组，分别给予补充浓度为0%、0.04%、0.16%和0.64%（重量/重量）NTs的饮食。治疗30天后，NTs可显著增加小鼠的强迫游泳时间，提高乳酸脱氢酶活性和肝糖原水平，并延缓小鼠血液尿素氮和血乳酸的积累。NTs还显著改善了疲劳诱导的氧化应激生物标志物和抗氧化酶的变化。值得注意的是，NTs增加了小鼠骨骼肌中的线粒体能量代谢酶活性。这些结果表明，NTs发挥抗疲劳作用，这可能归因于对氧化应激的抑制和骨骼肌线粒体功能的改善。NTs可作为一种新型天然剂用于缓解运动疲劳。：ATP：三磷酸腺苷；BLA：血乳酸；GSH-Px：谷胱甘肽过氧化物酶；LDH：乳酸脱氢酶；MDA：丙二醛；NTs：膳食核苷酸；SDH：琥珀酸脱氢酶；SOD：超氧化物歧化酶；BUN：血液尿素氮。
