外泌体环状 RNA hsa_circ_0006220 和 hsa_circ_0001666 作为胰腺癌诊断的生物标志物。
Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer.
机构信息
Department of Radiology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.
Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, China.
出版信息
J Clin Lab Anal. 2022 Jun;36(6):e24447. doi: 10.1002/jcla.24447. Epub 2022 Apr 21.
BACKGROUND
Pancreatic cancer is a highly malignant tumor of the digestive system.
OBJECTIVE
Exosomal circular RNA can be used as a biomarker for the early diagnosis of pancreatic cancer.
METHODS
The expression of various differentially expressed circRNAs in pancreatic cancer tissues was analyzed by gene chip, exosome expression was verified by electron microscopy and Western blotting, and the expression of exosomal circRNA in pancreatic cancer cells, tissues, and plasma were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
RESULTS
Compared with healthy controls, hsa_circ_0006220 and hsa_circ_0001666 were highly expressed in exosomes in the plasma of pancreatic cancer patients. The AUC values were 0.7817 for hsa_circ_0006220, 0.8062 for hsa_circ_0001666, and 0.884 for the combined diagnosis. In addition, clinicopathological features revealed that the expression of hsa_circ_0006220 in plasma exosomes from pancreatic cancer patients was associated with CA19-9 levels (p = 0.0001) and lymph node metastasis (p = 0.0005). The expression of hsa_circ_0001666 was correlated with both tumor size (p = 0.0157) and CA19-9 level (p = 0.0001).
CONCLUSIONS
The high expression of exosomal hsa_circ_0001666 and hsa_circ_0006220 suggests that these can be used as new biomarkers for the diagnosis and treatment of pancreatic cancer.
背景
胰腺癌是一种高度恶性的消化系统肿瘤。
目的
外泌体环状 RNA 可作为胰腺癌早期诊断的生物标志物。
方法
通过基因芯片分析胰腺癌组织中各种差异表达的 circRNA 的表达，电镜和 Western blot 验证外泌体表达，定量逆转录-聚合酶链反应（qRT-PCR）检测胰腺癌细胞、组织和血浆中外泌体 circRNA 的表达。
结果
与健康对照组相比，胰腺癌患者血浆中外泌体中 hsa_circ_0006220 和 hsa_circ_0001666 表达水平较高。hsa_circ_0006220 的 AUC 值为 0.7817，hsa_circ_0001666 的 AUC 值为 0.8062，联合诊断的 AUC 值为 0.884。此外，临床病理特征表明，胰腺癌患者血浆中外泌体中 hsa_circ_0006220 的表达与 CA19-9 水平（p = 0.0001）和淋巴结转移（p = 0.0005）相关。hsa_circ_0001666 的表达与肿瘤大小（p = 0.0157）和 CA19-9 水平（p = 0.0001）均相关。
结论
外泌体 hsa_circ_0001666 和 hsa_circ_0006220 的高表达表明，它们可作为胰腺癌诊断和治疗的新生物标志物。
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