School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371, Singapore.
Nanoscale Res Lett. 2011 Sep 30;6(1):535. doi: 10.1186/1556-276X-6-535.
The use of nanowires (NWs) for labeling, sensing, and sorting is the basis of detecting biomolecules attached on NWs by optical and magnetic properties. In spite of many advantages, the use of biomolecules-attached NWs sensing by photoelectrochemical (PEC) study is almost non-existent. In this article, the PEC study of dye-attached single-stranded DNA on Au NWs and Au-Ni-Au multilayer NWs prepared by pulse electrodeposition are investigated. Owing to quantum-quenching effect, the multilayer Au NWs exhibit low optical absorbance when compared with Au NWs. The tagged Au NWs show good fluorescence (emission) at 570 nm, indicating significant improvement in the reflectivity. Optimum results obtained for tagged Au NWs attached on functionalized carbon electrodes and its PEC behavior is also presented. A twofold enhancement in photocurrent is observed with an average dark current of 10 μA for Au NWs coated on functionalized sensing electrode. The importance of these PEC and optical studies provides an inexpensive and facile processing platform for Au NWs that may be suitable for biolabeling applications.
纳米线（NWs）在标记、传感和分类方面的应用是基于通过光学和磁性能检测附着在 NWs 上的生物分子的基础。尽管有许多优点，但通过光电化学（PEC）研究来检测附着生物分子的 NWs 的应用几乎是不存在的。本文研究了通过脉冲电沉积制备的金纳米线（Au NWs）和金-镍-金多层纳米线（Au-Ni-Au multilayer NWs）上附着的染料单链 DNA 的 PEC 研究。由于量子猝灭效应，与 Au NWs 相比，多层 Au NWs 的光吸收较低。标记的 Au NWs 在 570nm 处显示出良好的荧光（发射），表明反射率有了显著提高。还介绍了附着在功能化碳电极上的标记 Au NWs 的最佳结果及其 PEC 行为。在功能化传感电极上涂覆的 Au NWs 的光电流增加了两倍，平均暗电流为 10μA。这些 PEC 和光学研究的重要性为 Au NWs 提供了一个廉价且简便的处理平台，可能适用于生物标记应用。
