Department of Pediatrics, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
Chin Med. 2011 Sep 30;6:35. doi: 10.1186/1749-8546-6-35.
Peanut allergy is characterized by increased levels of peanut-specific IgE in the serum of most patients. Thus, the most logical therapy would be to inhibit the IgE production by committed B-cells. This study aims to investigate the unreported anti-IgE effects of Chinese herbal extracts of Rubia cordifolia (Qiancao) and Dianthus superbus (Qumai).
Seventy herbal extracts were tested for their ability to reduce IgE secretion by a human B-cell line. Those with the lowest inhibitory concentration 50 (IC50) values were tested in a mouse model of peanut-anaphylaxis. Anaphylactic scores, body temperature, plasma histamine and peanut-specific-immunoglobulins were determined.
Rubia cordifolia and Dianthus superbus inhibited the in vitro IgE production by a human B-cell line in a dose-dependent manner and the in vivo IgE production in a murine model of peanut allergy without affecting peanut-specific-IgG1 levels. After challenge, all mice in the sham groups developed anaphylactic reactions and increased plasma histamine levels. The extract-treated mice demonstrated significantly reduced peanut-triggered anaphylactic reactions and plasma histamine levels.
The extracts of Rubia cordifolia and Dianthus superbus inhibited the IgE production in vivo and in vitro as well as reduced anaphylactic reactions in peanut-allergic mice, suggesting potentials for allergy treatments.
花生过敏的特点是大多数患者血清中的花生特异性 IgE 水平升高。因此，最合理的治疗方法是抑制致敏 B 细胞的 IgE 产生。本研究旨在研究茜草（茜草）和瞿麦（瞿麦）的中草药提取物未报告的抗 IgE 作用。
测试了 70 种草药提取物抑制人 B 细胞系 IgE 分泌的能力。用花生过敏的小鼠模型测试 IC50 值最低的那些。测定过敏评分、体温、血浆组织胺和花生特异性免疫球蛋白。
茜草和瞿麦提取物以剂量依赖的方式抑制体外人 B 细胞系 IgE 的产生，以及花生过敏小鼠体内 IgE 的产生，而不影响花生特异性 IgG1 水平。在挑战后，所有假手术组的小鼠均发生过敏反应并增加血浆组织胺水平。经提取物处理的小鼠表现出明显减轻的花生触发的过敏反应和血浆组织胺水平。
茜草和瞿麦提取物抑制体内和体外的 IgE 产生，并减少花生过敏小鼠的过敏反应，提示其具有过敏治疗潜力。
