Department of Microbiology, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, Heilongjiang Province 150081, China.
Virus Res. 2012 Jan;163(1):197-201. doi: 10.1016/j.virusres.2011.09.029. Epub 2011 Sep 22.
To investigate the mechanism and prognosis of occult hepatitis B virus (HBV) infection (OBI) at a molecular level among healthy young adults, the presence of HBV DNA in 1176 sera samples collected from healthy young people after neonatal vaccination was assessed by nested polymerase chain reaction (PCR) using specific primers designed for the X and S regions of the HBV genome. Full-length HBV DNA from 9 patients with OBI (OB1-OB9) was cloned and sequenced. Deletions in the pre-S, basal core promoter (BCP), core (C) and polymerase (P) regions were observed. The data indicate that there is still a substantial risk of OBI in China despite neonatal vaccination. All deletions that were observed in the pre-S, BCP, C and P regions play a direct or indirect role in OBI. The presence of a deletion mutation in the pre-S1 region was considered to play a pivotal role in hepatocarcinogenesis and was found to increase the risk of hepatocellular carcinoma in the cohorts studied.
为了从分子水平上研究隐匿性乙型肝炎病毒（HBV）感染（OBI）在健康年轻成年人中的机制和预后，采用针对 HBV 基因组 X 和 S 区设计的特异性引物，通过巢式聚合酶链反应（PCR）检测了 1176 份来自新生儿接种后健康年轻人的血清样本中 HBV DNA 的存在情况。对 9 例 OBI 患者（OB1-OB9）的全长 HBV DNA 进行了克隆和测序。观察到前 S、基本核心启动子（BCP）、核心（C）和聚合酶（P）区的缺失。数据表明，尽管进行了新生儿接种，中国仍存在大量 OBI 风险。在前 S、BCP、C 和 P 区观察到的所有缺失均直接或间接影响 OBI。前 S1 区缺失突变的存在被认为在肝癌发生中起关键作用，并在研究队列中发现增加了肝细胞癌的风险。
