室内空气中的微球菌菌株的特性研究。
Characterization of Micrococcus strains isolated from indoor air.
机构信息
Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, Columbia, SC 29208, USA.
出版信息
Mol Cell Probes. 2012 Feb;26(1):1-5. doi: 10.1016/j.mcp.2011.09.003. Epub 2011 Sep 22.
Abstract
The characterization of microbes, such as opportunists and pathogens (e.g., methicillin resistant Staphylococcus aureus [MRSA]), in indoor air is important for understanding disease transmission from person-to-person. Common genera found in the human skin microbiome include Micrococcus and Staphylococcus, but there only a limited number of tests to differentiate these genera and/or species. Both genera are believed to be released into indoor air from the shedding of human skin and are morphologically difficult to distinguish. In the current work, after the extraction of proteins from micrococci and the separation of these proteins on one dimensional electrophoretic gels, tryptic peptides were analyzed by MALDI TOF MS and the mass profiles compared with those of a reference strain (ATCC 4698). The results confirmed that all strains were consistent in identity with Micrococcus luteus.
摘要
对微生物（如机会致病菌和病原体，例如耐甲氧西林金黄色葡萄球菌[MRSA]）的特性进行分析，对于了解人与人之间的疾病传播非常重要。在人类皮肤微生物组中常见的属包括微球菌属和葡萄球菌属，但目前只有少数几种方法可以区分这些属和/或种。这两个属都被认为是通过人体皮肤的脱落释放到室内空气中的，并且形态上难以区分。在本研究中，从微球菌属中提取蛋白质后，在一维电泳凝胶上分离这些蛋白质，然后用 MALDI-TOF MS 分析胰蛋白酶肽，并将质量谱与参考菌株（ATCC 4698）进行比较。结果证实所有菌株的身份与藤黄微球菌一致。
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