Solazzo Giulia, Rovelli Sabrina, Iodice Simona, Chung Matthew, Frimpong Michael, Bollati Valentina, Ferrari Luca, Ghedin Elodie
EPIGET LAB, Department of Clinical Science and Community Health, Università degli Studi di Milano, Milan, Italy.
Systems Genomics Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
bioRxiv. 2024 Aug 12:2024.08.12.607611. doi: 10.1101/2024.08.12.607611.
Air particulate matter (PM) is widely recognized for its potential to negatively affect human health, including changes in the upper respiratory microbiome. However, research on PM-associated microbiota remains limited and mostly focused on PM (e.g., PM and PM). This study aims to characterize for the first time the microbiome of Total Suspended Particles (TSP) and investigate the correlations of indoor TSP with the human upper respiratory microbiome. Biological and environmental samples were collected over three collection periods lasting three weeks each, between May and July 2022 at the University of Milan and the University of Insubria Como. TSP were sampled using a filter-based technique, while respiratory samples from both anterior nares (AN) and the nasopharynx (NP) were collected using swabs. Microbiome analysis of both human (N = 145) and TSP (N = 51) samples was conducted on metagenomic sequencing data. A comparison of indoor and outdoor TSP microbiomes revealed differences in microbial diversity and taxonomic composition. The indoor samples had higher relative abundance of environmental bacteria often associated with opportunistic infections like sp., as well as respiratory bacteria such as and . Additionally, both indoor and outdoor TSP samples contained broad spectrum antibiotic resistance genes. Indoor TSP exposure was negatively associated with commensal bacteria and positively associated with relative abundance. Finally, a correlation between the relative abundance of respiratory bacteria identified in the indoor TSP and the upper respiratory microbiome was found, suggesting a potential interaction between TSP and the upper airways.
空气颗粒物（PM）因其对人类健康产生负面影响的可能性而广为人知，包括对上呼吸道微生物群的改变。然而，关于与PM相关的微生物群的研究仍然有限，且大多集中在特定类型的PM（如PM和PM）上。本研究旨在首次对总悬浮颗粒物（TSP）的微生物群进行表征，并研究室内TSP与人类上呼吸道微生物群之间的相关性。在2022年5月至7月期间，于米兰大学和科莫因苏布里亚大学进行了三个为期三周的采集期，收集了生物和环境样本。TSP采用基于过滤器的技术进行采样，而前鼻孔（AN）和鼻咽（NP）的呼吸道样本则使用拭子收集。对人类（N = 145）和TSP（N = 51）样本的微生物群分析均基于宏基因组测序数据进行。室内和室外TSP微生物群的比较揭示了微生物多样性和分类组成的差异。室内样本中通常与机会性感染相关的环境细菌（如 菌属）以及呼吸道细菌（如 菌和 菌）的相对丰度较高。此外，室内和室外TSP样本均含有广谱抗生素抗性基因。室内TSP暴露与共生细菌呈负相关，与 菌的相对丰度呈正相关。最后，发现室内TSP中鉴定出的呼吸道细菌的相对丰度与上呼吸道微生物群之间存在相关性，这表明TSP与上呼吸道之间可能存在相互作用。
