Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, Ontario N1G 3W4, Canada.
Appl Environ Microbiol. 2011 Dec;77(23):8265-71. doi: 10.1128/AEM.05562-11. Epub 2011 Sep 30.
Campylobacter jejuni continues to be the leading cause of bacterial food-borne illness worldwide, so improvements to current methods used for bacterial detection and disease prevention are needed. We describe here the genome and proteome of C. jejuni bacteriophage NCTC 12673 and the exploitation of its receptor-binding protein for specific bacterial detection. Remarkably, the 135-kb Myoviridae genome of NCTC 12673 differs greatly from any other proteobacterial phage genome described (including C. jejuni phages CP220 and CPt10) and instead shows closest homology to the cyanobacterial T4-related myophages. The phage genome contains 172 putative open reading frames, including 12 homing endonucleases, no visible means of packaging, and a putative trans-splicing intein. The phage DNA appears to be strongly associated with a protein that interfered with PCR amplification and estimation of the phage genome mass by pulsed-field gel electrophoresis. Identification and analyses of the receptor-binding protein (Gp48) revealed features common to the Salmonella enterica P22 phage tailspike protein, including the ability to specifically recognize a host organism. Bacteriophage receptor-binding proteins may offer promising alternatives for use in pathogen detection platforms.
空肠弯曲菌仍然是全球细菌性食源性疾病的主要原因，因此需要改进目前用于细菌检测和疾病预防的方法。我们在这里描述了空肠弯曲菌噬菌体 NCTC 12673 的基因组和蛋白质组，以及利用其受体结合蛋白进行特异性细菌检测。值得注意的是，NCTC 12673 的 135kb 肌尾噬菌体基因组与任何其他已描述的变形菌噬菌体基因组（包括空肠弯曲菌噬菌体 CP220 和 CPt10）有很大的不同，而与蓝细菌 T4 相关的肌尾噬菌体最为相似。噬菌体基因组包含 172 个推定的开放阅读框，包括 12 个归巢内切酶、没有明显的包装方式和一个推定的转剪接内含肽。噬菌体 DNA 似乎与一种蛋白质强烈相关，这种蛋白质干扰了 PCR 扩增和脉冲场凝胶电泳估计噬菌体基因组的质量。受体结合蛋白（Gp48）的鉴定和分析揭示了与沙门氏菌 P22 噬菌体尾刺蛋白共同的特征，包括特异性识别宿主生物的能力。噬菌体受体结合蛋白可能为病原体检测平台的应用提供有前途的替代方案。
