Sarkar S, Nelson A J, Jones O W
J Med Genet. 1977 Aug;14(4):250-5. doi: 10.1136/jmg.14.4.250.
Sperm from normal human donors and a G6PD-A- individual were examined for x-linked glucose-6-phosphate dehydrogenase activity and autosomally linked 6-phosphogluconate dehydrogenase activity. With the use of fluorescence microscopy, we divised a procedure to visualise in individual sperm cells the fluorescence of reduced coenzyme NADPH formed by each of the two enzymes in the presence of appropriate substrates. We found significant differences in the population distribution of sperm expressing each of the two activities, and the ratio of the two activities in sperm homogenate is very different from the one found in erythrocyte lysates. The possibility of haploid gene expression has been considered in interpreting these results.
对来自正常人类供体和一名G6PD - A -个体的精子进行了X连锁葡萄糖 - 6 -磷酸脱氢酶活性和常染色体连锁6 -磷酸葡萄糖酸脱氢酶活性检测。利用荧光显微镜，我们设计了一种方法，以在单个精子细胞中可视化两种酶在适当底物存在下形成的还原型辅酶NADPH的荧光。我们发现表达两种活性中每一种的精子群体分布存在显著差异，并且精子匀浆中两种活性的比率与红细胞裂解物中的比率非常不同。在解释这些结果时考虑了单倍体基因表达的可能性。
