Medina-Puerta M M, Gallego-Iniesta M, Garrido-Pertierra A
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Rev Esp Fisiol. 1988 Dec;44(4):433-9.
The influence in the activities of the glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase produced by the ratio changes NADPH/NADP is studied. The intracellular ratios of 20 and 10 are enough to achieve total inhibition of these two enzymes, respectively. Measurements of a number of metabolic intermediates show that the concentrations of Krebs cycle compounds are higher than those of glycolytic pathway metabolites. From a consideration of these values, the regulation of the pentose phosphate cycle mainly by the intracellular NADPH/NADP ratio, is discussed.
研究了NADPH/NADP比值变化对葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶活性的影响。细胞内比值分别为20和10时足以实现对这两种酶的完全抑制。对多种代谢中间体的测量表明，三羧酸循环化合物的浓度高于糖酵解途径代谢物的浓度。基于这些数值，讨论了磷酸戊糖途径主要通过细胞内NADPH/NADP比值进行的调节。
