Servicio de Microbiología and CIBER en Epidemiología y Salud Pública (CIBERESP), Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain.
J Cyst Fibros. 2012 Jan;11(1):59-62. doi: 10.1016/j.jcf.2011.09.001. Epub 2011 Oct 2.
Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n=182) recovered from CF patients over a 15 year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database.
从囊性纤维化 (CF) 患者中鉴定非发酵革兰氏阴性菌 (NFGNB) 通常受到限制。对在 15 年期间从 CF 患者中回收的一组储存的 NFGNB 分离株 (n=182) 进行了检查。比较了在此期间常规报告的鉴定与 MALDI-TOF MS 获得的鉴定。在属水平上给出不一致鉴定的分离株通过 16S rDNA 测序进一步分析。MALDI-TOF MS 系统鉴定了 94%的分离株，包括伯克霍尔德菌和 Pandoraea 属分离株，后者以前通过常规微生物方法错误鉴定为其他 NFGNB。MALDI-TOF MS 无法鉴定与数据库中没有条目有关。
