Levin D E, Blunt E L, Levin R E
Mutat Res. 1981 Oct;85(5):309-21. doi: 10.1016/0165-1161(81)90222-3.
A modified fluctuation test (Green) with the Ames' tester strain Salmonella typhimurium TA98 has been examined for sensitivity to histidine feeding and for detection of minimal concentrations of daunomycin and S9 activated benzo[a]pyrene. The fluctuation test was found operable over a range of histidine concentrations between 0.25 and 1.25 microgram/ml using 48 tube assays and microtitre plates with 120 wells. The agar plate method yielded a comparable operational range for histidine concentration. With daunomycin, the microtiter fluctuation test was 48-fold greater in sensitivity than the macroscale fluctuation test. With benzo [a] pyrene, the microtiter fluctuation test was 4.8-fold greater in sensitivity than the macroscale test. The microtiter assay was 2.4 and 2.5-fold more sensitive than the plate and treat method with daunomycin and benzo [a] pyrene respectively.
已采用改良的波动试验（格林法），以鼠伤寒沙门氏菌TA98作为艾姆斯试验菌株，检测其对组氨酸添加的敏感性以及对最低浓度柔红霉素和经S9活化的苯并[a]芘的检测能力。结果发现，使用48管试验以及120孔微量滴定板时，波动试验在0.25至1.25微克/毫升的组氨酸浓度范围内均可操作。琼脂平板法得到了类似的组氨酸浓度操作范围。对于柔红霉素，微量滴定波动试验的灵敏度比宏观波动试验高48倍。对于苯并[a]芘，微量滴定波动试验的灵敏度比宏观试验高4.8倍。对于柔红霉素和苯并[a]芘，微量滴定试验分别比平板及处理法灵敏2.4倍和2.5倍。
