新型一氧化氮供体Rut-bpy（顺式-[Ru(bpy)2(SO3)(NO)]PF 6）对L-NAME诱导的大鼠高血压的影响。

Effects of Rut-bpy (Cis-[Ru(bpy)2(SO3)(NO)]PF 6), a novel nitric oxide donor, in L-NAME-induced hypertension in rats.

作者信息

Campelo Marcio Wilker Soares, Campelo Ana Paula Bomfim Soares, Lopes Luiz Gonzaga de França, Santos Armenio Aguiar Dos, Guimarães Sergio Botelho, Vasconcelos Paulo Roberto Leitão de

机构信息

Department of Surgery, Federal University of Ceara, Fortaleza-CE, Brazil.

出版信息

Acta Cir Bras. 2011;26 Suppl 1:57-9. doi: 10.1590/s0102-86502011000700012.

DOI:10.1590/s0102-86502011000700012

PMID:21971659

Abstract

PURPOSE

To evaluate the effect of Rut-bpy (Cis-[Ru(bpy)2(SO3)(NO)]PF 6), a novel nitric oxide donor in Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats.

METHODS

Twenty-four male Wistar rats were randomly assigned to four groups (n=6), named according to the treatment applied (G1-Saline, G2-Rut-bpy, G3-L-NAME and G4-L-NAME+Rut-bpy). L-NAME (30 mg/Kg) was injected intraperitoneally 30 minutes before the administration of Rut-bpy (100 mg/Kg). Mean abdominal aorta arterial blood pressure (MAP) was continuously monitored.

RESULTS

Mean arterial blood pressure (MAP) in G3 rats rose progressively, reaching 147±16 mmHg compared with 100±19 mm Hg in G1 rats (p<0.05). In G4 rats, treated with L-NAME+Rut-bpy, MAP reached 149+11 mm Hg while in G2 rats, treated with Rut-bpy, MAP values were 106±11 mm Hg. In G1 rats these values decreased progressively reaching 87+14 mm Hg after 30 minutes. An important finding was the maintenance of the MAP throughout the experiment in G2 rats.

CONCLUSION

Rut-bpy does not decrease the MAP in L-Name induced hypertensive rats. However, when it is used in anesthetized hypotensive rats a stable blood pressure is obtained.

摘要

目的

评估新型一氧化氮供体顺式-[钌（联吡啶）2（亚硫酸根）（一氧化氮）]六氟磷酸酯（Rut-bpy）对Nω-硝基-L-精氨酸甲酯（L-NAME）诱导的高血压大鼠的作用。

方法

将24只雄性Wistar大鼠随机分为四组（n = 6），根据所施加的处理命名（G1-生理盐水，G2-Rut-bpy，G3-L-NAME和G4-L-NAME + Rut-bpy）。在给予Rut-bpy（100 mg/Kg）前30分钟腹腔注射L-NAME（30 mg/Kg）。连续监测平均腹主动脉动脉血压（MAP）。

结果

G3组大鼠的平均动脉血压（MAP）逐渐升高，达到147±16 mmHg，而G1组大鼠为100±19 mmHg（p<0.05）。在接受L-NAME + Rut-bpy治疗的G4组大鼠中，MAP达到149 + 11 mmHg，而在接受Rut-bpy治疗的G2组大鼠中，MAP值为106±11 mmHg。在G1组大鼠中，这些值逐渐下降，30分钟后降至87 + 14 mmHg。一个重要的发现是G2组大鼠在整个实验过程中MAP保持稳定。