Mattos Cinara de Cássia Brandão de, Spegiorin Lígia Cosentino Junqueira Franco, Meira Cristina da Silva, Silva Thaís da Costa, Ferreira Ana Iara da Costa, Nakashima Fabiana, Pereira-Chioccola Vera Lúcia, Mattos Luiz Carlos de
Immunogenetics Laboratory, Department of Molecular Biology, São José do Rio Preto, Brazil.
Sao Paulo Med J. 2011;129(4):261-6. doi: 10.1590/s1516-31802011000400010.
Toxoplasmosis transmission during pregnancy can cause severe sequelae in fetuses and newborns. Maternal antibodies may be indicators of risk or immunity. The aim here was to evaluate seropositivity for anti-Toxoplasma gondii (anti-T. gondii) immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies and IgG avidity in pregnant women and their newborn infants.
Cross-sectional study in a high-risk pregnancy outpatient clinic.
Serum samples from pregnant women (n = 87) and their respective newborns (n = 87) were evaluated for anti-T. gondii antibodies using indirect immunofluorescence (IIF) (IgM and IgG), enzyme-linked immunosorbent assay (ELISA) (IgG) and an avidity test.
Anti-T. gondii antibodies were identified in 64.4% of the serum samples from the mothers and their infants (56/87). Except for two maternal serum samples (2.3%), all others were negative for anti-T. gondii IgM antibodies, using IIF. The results showed that 92.9% of the pregnant women had high IgG avidity indexes (> 30%) and four samples had avidity indexes between 16 and 30%. Two women in the third trimester of pregnancy were positive for anti-T. gondii IgM antibodies; their babies had avidity indexes between 16 and 30%. The avidity indexes of serum from the other 83 newborns were similar to the results from their mothers.
The results showed that 2% of the pregnant women were at risk of T. gondii transmission during the gestational period. These data seem to reflect the real situation of gestational toxoplasmosis in the northwestern region of the state of São Paulo.
孕期弓形虫传播可导致胎儿和新生儿出现严重后遗症。母体抗体可能是风险或免疫力的指标。本研究旨在评估孕妇及其新生儿抗弓形虫免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗体的血清阳性率以及IgG亲和力。
在高危妊娠门诊进行的横断面研究。
使用间接免疫荧光法（IIF）（检测IgM和IgG）、酶联免疫吸附测定法（ELISA）（检测IgG）和亲和力试验，对87名孕妇及其各自新生儿的血清样本进行抗弓形虫抗体检测。
在母亲及其婴儿的64.4%（56/87）血清样本中检测到抗弓形虫抗体。除两份母亲血清样本（2.3%）外，其余所有样本经IIF检测抗弓形虫IgM抗体均为阴性。结果显示，92.9%的孕妇IgG亲和力指数较高（> 30%），四份样本的亲和力指数在16%至30%之间。两名孕晚期妇女抗弓形虫IgM抗体呈阳性；其婴儿的亲和力指数在16%至30%之间。其他83名新生儿血清的亲和力指数与其母亲的结果相似。
结果表明，2%的孕妇在孕期有弓形虫传播风险。这些数据似乎反映了圣保罗州西北部地区孕期弓形虫病的实际情况。
