Faculty of Dentistry, McGill University, Montreal, Canada.
Oral Dis. 2012 Mar;18(2):162-8. doi: 10.1111/j.1601-0825.2011.01858.x. Epub 2011 Oct 5.
Experimental approaches tested to date for functional restoration of salivary glands (SGs) are tissue engineering, gene transfer, and cell therapy. To further develop these therapies, identifying specific cell surface markers for the isolation of salivary acinar cells is needed. To test a panel of cell surface markers [used in the isolation of mesenchymal stem cells, (MSCs)] for the localization of salivary acinar cells.
Human submandibular and parotid glands were immunostained with a panel of MSC markers and co-localized with salivary acinar cell differentiation markers [α-amylase, Na-K-2Cl cotransporter-1, aquaporin-5 (AQP5)]. Additional cell markers were also used, such as α-smooth muscle actin (to identify myoepithelial cells), cytokeratin-5 (basal ductal cells), and c-Kit (progenitor cells).
CD44 identified serous acini, while CD166 identified mucous acini. Cytokeratin-5 identified basal duct cells and 50% of myoepithelial cells. None of the remaining cell surface markers (Stro-1, CD90, CD106, CD105, CD146, CD19, CD45, and c-Kit) were expressed in any human salivary cell.
CD44 and CD166 localized human salivary serous and mucous acinar cells, respectively. These two cell surface markers will be useful in the isolation of specific populations of salivary acinar cells.
迄今为止，用于唾液腺（SGs）功能恢复的实验方法包括组织工程、基因转移和细胞疗法。为了进一步开发这些疗法，需要确定用于分离唾液腺泡细胞的特定细胞表面标志物。测试一组细胞表面标志物[用于分离间充质干细胞（MSCs）]，以定位唾液腺泡细胞。
用人下颌下腺和腮腺的一组 MSC 标志物进行免疫染色，并与唾液腺泡细胞分化标志物[α-淀粉酶、Na-K-2Cl 共转运蛋白-1、水通道蛋白 5（AQP5）]共定位。还使用了其他细胞标志物，如α-平滑肌肌动蛋白（识别肌上皮细胞）、细胞角蛋白-5（基底导管细胞）和 c-Kit（祖细胞）。
CD44 鉴定出浆液性腺泡，而 CD166 鉴定出粘液性腺泡。细胞角蛋白-5 鉴定出基底导管细胞和 50%的肌上皮细胞。其余的细胞表面标志物（Stro-1、CD90、CD106、CD105、CD146、CD19、CD45 和 c-Kit）均未在任何人类唾液细胞中表达。
CD44 和 CD166 分别定位人唾液浆液性腺泡和粘液性腺泡。这两个细胞表面标志物将有助于分离特定的唾液腺泡细胞群体。
