State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Beijing 102206, China.
J Virol. 2011 Dec;85(24):13432-8. doi: 10.1128/JVI.05266-11. Epub 2011 Oct 5.
Human infections of H5N1 highly pathogenic avian influenza virus have continued to occur in China without corresponding outbreaks in poultry, and there is little conclusive evidence of the source of these infections. Seeking to identify the source of the human infections, we sequenced 31 H5N1 viruses isolated from humans in China (2005 to 2010). We found a number of viral genotypes, not all of which have similar known avian virus counterparts. Guided by patient questionnaire data, we also obtained environmental samples from live poultry markets and dwellings frequented by six individuals prior to disease onset (2008 and 2009). H5N1 viruses were isolated from 4 of the 6 live poultry markets sampled. In each case, the genetic sequences of the environmental and corresponding human isolates were highly similar, demonstrating a link between human infection and live poultry markets. Therefore, infection control measures in live poultry markets are likely to reduce human H5N1 infection in China.
在中国，人感染 H5N1 高致病性禽流感病毒的情况持续发生，但家禽中并未出现相应的疫情，这些感染的源头也几乎没有确凿的证据。为了确定人感染的源头，我们对 2005 年至 2010 年期间从中国感染 H5N1 病毒的 31 人进行了病毒测序。我们发现了一些病毒基因型，并非所有基因型都有类似的已知禽流感病毒对应物。根据患者问卷调查数据，我们还从六个在发病前（2008 年和 2009 年）经常光顾活禽市场和住所的个体获得了环境样本。从 6 个活禽市场中的 4 个采集到了 H5N1 病毒。在每种情况下，环境和相应人类分离株的遗传序列都非常相似，证明了人类感染与活禽市场之间存在联系。因此，活禽市场的感染控制措施可能会降低中国的人感染 H5N1 风险。
