Leoni P, Carli F, Halliday D
Nutrition Research Group, Clinical Research Centre, Harrow, Middlesex, U.K.
Biochem J. 1990 Jul 1;269(1):31-4. doi: 10.1042/bj2690031.
The intermediate filament proteins desmin and vimentin from pregnant and non-pregnant uterine muscle and smooth-muscle cells in culture were analysed using SDS/PAGE. The desmin content in uterine muscle increases dramatically during pregnancy, whereas vimentin remains unchanged or changes very little. When muscle cells are kept in culture, a considerable increase in vimentin content is observed as compared with vimentin in freshly isolated non-pregnant uterine tissue. Our results strengthen the view that vimentin and desmin filaments have independent function and turnover, and point to a predominantly structural role for desmin filaments.
利用十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS/PAGE）分析了妊娠和未妊娠子宫肌肉以及培养的平滑肌细胞中的中间丝蛋白结蛋白和波形蛋白。子宫肌肉中的结蛋白含量在妊娠期间显著增加，而波形蛋白则保持不变或变化很小。当肌肉细胞进行培养时，与新鲜分离的未妊娠子宫组织中的波形蛋白相比，波形蛋白含量有相当大的增加。我们的结果强化了这样一种观点，即波形蛋白丝和结蛋白丝具有独立的功能和更新，并表明结蛋白丝主要起结构作用。
