Heo Chaejeong, Hong Min Young, Jo Areum, Lee Young Hee, Suh Minah
Department of Energy Science, Department of Physics, Sungkyunkwan University, Suwon, Korea.
J Acupunct Meridian Stud. 2011 Sep;4(3):198-202. doi: 10.1016/j.jams.2011.09.009. Epub 2011 Oct 1.
A melanoma tumor is a representative malignant tumor. Melanoma tumor growth involves vigorous angiogenesis around the tumor and a vasculogenic-like network inside an aggressive tumor. Primo vessels (PVs) are also found on the surface of the tumor and coexist alongside blood vessels (BVs), and sometimes within the BVs. We hypothesized that the primo vessels system plays a significant role in regulating the development of a melanoma tumor, and therefore has a tight coupling with BVs and angiogenesis. To prove this hypothesis, we developed a murine melanoma model by inoculating melanoma cell lines into the abdominal region. We used a green fluorescent protein (GFP) expressing mouse as a host to distinguish the endogenous source of the tumor PVs. We found strong formation of PVs on the tumor that coexisted with BVs and expression of GFP. PVs also had a tight coupling with adipose tissues, especially with white adipose tissue. These data suggest that the PVs of an induced melanoma tumor evolve endogenously from the host body and may be highly related to BVs and adipose tissue. This model of PVs in an overexpressing GFP mouse is a useful system for observing PVs, primo nodes, and primo vessel networks, and has potential to be developed as a model for examining novel treatments for cancer metastasis.
黑色素瘤是一种典型的恶性肿瘤。黑色素瘤的生长涉及肿瘤周围活跃的血管生成以及侵袭性肿瘤内部类似血管生成的网络。在肿瘤表面也发现了初级血管（PVs），它们与血管（BVs）共存，有时还存在于血管内部。我们假设初级血管系统在调节黑色素瘤的发展中起重要作用，因此与血管和血管生成紧密相关。为了验证这一假设，我们通过将黑色素瘤细胞系接种到腹部区域建立了小鼠黑色素瘤模型。我们使用表达绿色荧光蛋白（GFP）的小鼠作为宿主，以区分肿瘤初级血管的内源性来源。我们发现肿瘤上有大量与血管共存且表达绿色荧光蛋白的初级血管形成。初级血管还与脂肪组织紧密相关，尤其是白色脂肪组织。这些数据表明，诱导性黑色素瘤肿瘤的初级血管是从宿主体内内生演化而来的，可能与血管和脂肪组织高度相关。这种在过表达绿色荧光蛋白小鼠中的初级血管模型是观察初级血管、初级节点和初级血管网络的有用系统，并且有潜力被开发为研究癌症转移新疗法的模型。
