INRA, UMR1128 Génétique et Microbiologie, F-54506 Vandœuvre-lès-Nancy Cedex, France.
BMC Microbiol. 2011 Oct 7;11:223. doi: 10.1186/1471-2180-11-223.
Streptococcus thermophilus is an important starter strain for the production of yogurt and cheeses. The analysis of sequenced genomes of four strains of S. thermophilus indicates that they contain several genes of the rgg familly potentially encoding transcriptional regulators. Some of the Rgg proteins are known to be involved in bacterial stress adaptation.
In this study, we demonstrated that Streptococcus thermophilus thermal stress adaptation required the rgg0182 gene which transcription depends on the culture medium and the growth temperature. This gene encoded a protein showing similarity with members of the Rgg family transcriptional regulator. Our data confirmed that Rgg0182 is a transcriptional regulator controlling the expression of its neighboring genes as well as chaperones and proteases encoding genes. Therefore, analysis of a Δrgg0182 mutant revealed that this protein played a role in the heat shock adaptation of Streptococcus thermophilus LMG18311.
These data showed the importance of the Rgg0182 transcriptional regulator on the survival of S. thermophilus during dairy processes and more specifically during changes in temperature.
嗜热链球菌是酸奶和奶酪生产的重要起始菌株。对 4 株嗜热链球菌测序基因组的分析表明，它们含有几个 rgg 家族的基因，这些基因可能编码转录调节剂。一些 Rgg 蛋白已知参与细菌应激适应。
在本研究中，我们证明了嗜热链球菌的热应激适应需要 rgg0182 基因，该基因的转录取决于培养基和生长温度。该基因编码的蛋白与 Rgg 家族转录调节剂的成员具有相似性。我们的数据证实，Rgg0182 是一种转录调节剂，它控制其邻近基因以及伴侣蛋白和蛋白酶编码基因的表达。因此，对Δrgg0182 突变体的分析表明，该蛋白在嗜热链球菌 LMG18311 的热休克适应中发挥作用。
这些数据表明，Rgg0182 转录调节剂对嗜热链球菌在乳制品加工过程中，特别是在温度变化过程中的生存至关重要。
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