School of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Material, Minister of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China.
Int J Pharm. 2011 Dec 15;421(2):310-20. doi: 10.1016/j.ijpharm.2011.09.033. Epub 2011 Oct 1.
Poly(ɛ-caprolactone)/multi-walled carbon nanotubes (PCL/MWCNTs) composite nanofibers with various content of green tea polyphenols (GTP) were successfully fabricated via an electrospinning technology to maintain the chemical structural stability of GTP. The non-covalent interaction between MWCNTs and GTP was measured by UV-vis spectrophotometer and FT-IR. The topographical features of the nanofibers were characterized by scanning electron microscopy (SEM). The dispersibility of MWCNTs and the distribution of GTP in nanofibers were observed by transmission electron microscopy (TEM) and laser scanning confocal microscope (LSCM), respectively. In vitro degradation was also characterized in terms of the morphological change and the mass loss of the nanofiber meshes. In vitro GTP release behavior was investigated in phosphate-buffered solution (PBS) at 37°C. Alamar blue assays were performed to estimate the cytotoxicity of the nanofibers with normal osteoblast cells and the antiproliferative effects to A549 and Hep G2 tumor cells. The results exhibited that the GTP-loaded composite nanofibers possessed a significant inhibition effect to tumor cells. Therefore, GTP, as a multifunctional drug, encapsulated into polymer composite nanofibers, must have broad application prospects in cancer therapy.
聚（己内酯）/多壁碳纳米管（PCL/MWCNTs）复合纳米纤维，其含有不同含量的绿茶多酚（GTP），通过静电纺丝技术成功制备，以保持 GTP 的化学结构稳定性。通过紫外可见分光光度计和傅里叶变换红外光谱仪测量 MWCNTs 和 GTP 之间的非共价相互作用。扫描电子显微镜（SEM）用于表征纳米纤维的形貌特征。通过透射电子显微镜（TEM）和激光共聚焦显微镜（LSCM）观察 MWCNTs 的分散性和 GTP 在纳米纤维中的分布。还通过纳米纤维网的形态变化和质量损失来研究体外降解。在 37°C 的磷酸盐缓冲溶液（PBS）中研究了体外 GTP 释放行为。通过 Alamar blue 测定法评估纳米纤维对正常成骨细胞的细胞毒性和对 A549 和 Hep G2 肿瘤细胞的增殖抑制作用。结果表明，载有 GTP 的复合纳米纤维对肿瘤细胞具有显著的抑制作用。因此，作为一种多功能药物的 GTP 被包裹在聚合物复合纳米纤维中，在癌症治疗中必将具有广阔的应用前景。
