State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Appl Microbiol Biotechnol. 2012 Mar;93(5):2147-59. doi: 10.1007/s00253-011-3608-5. Epub 2011 Oct 9.
Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate synthase, 2,3-butanediol dehydrogenase, and a LysR-like regulator, respectively. Unlike in Klebsiella sp. or Klebsiella pneumonie and Vibrio sp. or Vibrio cholerae, the gene slaC is separated from other genes. Then it showed that two regulators, SwrR and SlaR, are in charge of this process by exerting effect on the transcription of genes slaA and slaB. By contrast, the expression of gene slaC is unaffected by the two regulators. It means that these two regulators affect the production of 2,3-butanediol by regulating the production of acetoin. Based on these findings, we successfully accelerated the 2,3-butanediol production by inactivation of gene swrR. The obtained results and further investigations should lead to a more suitable fermentation strategy and strain improvement which would be applicable to the industrial production of 2,3-butanediol.
粘质沙雷氏菌因其产量高、生产效率高等优势，已被证明是一种有潜力的工业 2,3-丁二醇生产菌株。在本研究中，成功克隆了 slaA、slaB、slaC 和 slaR 基因，并进一步证实它们分别编码乙酰乳酸脱羧酶、乙酰乳酸合酶、2,3-丁二醇脱氢酶和一个 LysR 样调控因子。与克雷伯氏菌属或肺炎克雷伯菌和弧菌属或霍乱弧菌不同，slaC 基因与其他基因分离。结果表明，两个调控因子 SwrR 和 SlaR 通过对 slaA 和 slaB 基因的转录发挥作用来控制这一过程。相比之下，基因 slaC 的表达不受这两个调控因子的影响。这意味着这两个调控因子通过调节乙酰丁酮的产生来影响 2,3-丁二醇的生产。基于这些发现，我们通过失活基因 swrR 成功加速了 2,3-丁二醇的生产。这些研究结果和进一步的研究应该会导致更适合 2,3-丁二醇工业生产的发酵策略和菌株改良。
